Sino Biological/MMP-2 Protein, Human, Recombinant/1/10082-HNAH
商品编号：
10082-HNAH
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
MMP-2 Protein, Human, Recombinant: Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is > 1,000 pmoles/min/µg.2. Measured by its binding ability in a functional ELISA.3. Immobilized human MMP2 at 10 μg/mL (100 μl/well) can bind human TIMP2/Fc (Cat: 10396-H01H). The EC50 of human TIMP2/Fc is 0.02 μg/mL.(Activation description: The proenzyme needs to be activated by APMA for an activated form)
Protein Construction
A DNA sequence encoding the native human MMP2 (NP_004521.1) (Met 1-Cys 660) was expressed and purified.
Accession#
NP_004521.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 30
Molecule Mass
The recombinant human MMP2 consists of 631 amino acids and migrates as an 72 kDa band as predicted in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 0.05 % Brij-35, 150 mM NaCl, 5 mM CaCl2, 50 mM Tris, pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
MMP-2 Protein, Human, Recombinant: Images
Measured by its binding ability in a functional ELISA. Immobilized human MMP2 at 10 μg/mL (100 μl/well) can bind human TIMP2/Fc (Cat: 10396-H01H). The EC50 of human TIMP2/Fc is 0.02 μg/mL.
MMP-2 Protein, Human, Recombinant: Synonyms
CLG4 Protein, Human; CLG4A Protein, Human; MMP-2 Protein, Human; MMP-II Protein, Human; MONA Protein, Human; TBE-1 Protein, Human
MMP-2 Background Information
Matrix Metalloproteinase-2 (MMP-2) is an enzyme that degrades components of the extracellular matrix and thus plays a pivotal role in cell migration during physiological and pathological processes. MMP-2 expression is dependent on extracellular matrix metalloproteinase inducer (EMMPRIN), Her2/neu, growth factors, cytokines, and hormones. Pro-MMP-2 activation needs MT1-MMP and TIMP-2 contribution. MMP-2 is changed in distribution and increased in amount in the ventral cochlear nucleus after unilateral cochlear ablation. A low level of MMP-2 is linked to a favorable prognosis in patients with a hormone receptor-negative tumor, usually associated with high risk. As a zymogen requiring proteolytic activation for catalytic activity, MMP-2 has been implicated broadly in the invasion and metastasis of many cancer model systems, including human breast cancer (HBC). Blocking MMP-2 secretion and activation during breast carcinoma development may decrease metastasis. The detection of active MMP-2 alone or the rate of pro-MMP-2 and active MMP-2 is considered a very sensitive indicator of cancer metastasis. Modulation of MMP-2 expression and activation through specific inhibitors and activators may thus provide a new mechanism for breast cancer treatment.
Full Name
matrix metallopeptidase 2
Research Areas
- Cancer Drug Targets
References
- Thompson EW, et al. (1994) Collagen induced MMP-2 activation in human breast cancer. Breast Cancer Res Treat. 31(2-3): 357-70.
- Jezierska A, et al. (2009) Matrix metalloproteinase-2 involvement in breast cancer progression: a mini-review. Med Sci Monit. 15(2): RA32-40.
- Fredrich M, et al. (2010) MMP-2 is involved in synaptic remodeling after cochlear lesion. Neuroreport. 21(5): 324-7.
Mouse MMP-2 Proteins
- MMP-2 Protein, Mouse, Recombinant (His Tag)52740-M07H
- A graphene oxide-based FRET sensor for rapid and sensitive detection of matrix metalloproteinase 2 in human serum sampleAuthorSong, E;Cheng, D;Song, Y;Jiang, M;Yu, J;Wang, Y;Year2013JournalBiosens BioelectronApplicationenzymePubMed ID: 23623988FoldedExpand
- Enzyme-responsive liposomes modified adenoviral vectors for enhanced tumor cell transduction and reduced immunogenicityAuthorWan, Y;Han, J;Fan, G;Zhang, Z;Gong, T;Sun, X;Year2013JournalBiomaterialsPubMed ID: 23360783FoldedExpand
- Fiber-optic protease sensor based on the degradation of thin gelatin filmsAuthorSchyrr, B;Boder-Pasche, S;Ischer, R;Smajda, R;Year2014JournalSensing and Bio-Sensing ResearchApplicationenzymeFoldedExpand
- Bio-inspired polymer envelopes around adenoviral vectors to reduce immunogenicity and improve in vivo kineticsAuthorFan, G;Fan, M;Wang, Q;Jiang, J;Wan, Y;Gong, T;Zhang, Z;Sun, X;Year2015JournalActa BiomaterApplicationenzyme (control)PubMed ID: 26546972FoldedExpand
- Laminin Promotes Metalloproteinase Mediated Dystroglycan Processing to Regulate Oligodendrocyte Progenitor Cell ProliferationAuthorLeiton, CV;Aranmolate, A;Eyermann, C;Menezes, MJ;Escobar-Hoyos, LF;Husain, S;Winder, SJ;Colognato, H;Year2015JournalJ. Neurochem.ApplicationenzymePubMed ID: 26171643FoldedExpand
- Modular Design and Facile Synthesis of Enzyme-Responsive Peptide-Linked Block Copolymers for Efficient Delivery of DoxorubicinAuthorKe, W;Li, J;Zhao, K;Zha, Z;Han, Y;Wang, Y;Yin, W;Zhang, P;Ge, Z;Year2016JournalBiomacromoleculesApplicationenzymePubMed ID: 27564064FoldedExpand
- Activatable Near-Infrared Probe for Fluorescence Imaging of γ-Glutamyl Transpeptidase in Tumor Cells and In VivoAuthorLuo, Z;Feng, L;An, R;Duan, G;Yan, R;Shi, H;He, J;Zhou, Z;Ji, C;Chen, HY;Ye, D;Year2017JournalChemistryApplicationcontrol (negative)PubMed ID: 28653778FoldedExpand
- Peptide Microarray-Based Metal Enhanced Fluorescence Assay for Multiple Profiling of Matrix Metalloproteinases ActivitiesAuthorLei, Z;Zhang, H;Wang, Y;Meng, X;Wang, Z;Year2017JournalAnal. Chem.Applicationenzyme(FRET-Peptide Microarray-Based MEF Assay)PubMed ID: 28516765FoldedExpand
- Smart Asymmetric Vesicles with Triggered Availability of Inner Cell-Penetrating Shells for Specific Intracellular Drug DeliveryAuthorLi, J;Xiao, S;Xu, Y;Zuo, S;Zha, Z;Ke, W;He, C;Ge, Z;Year2017JournalACS Appl Mater InterfacesApplicationenzyme（cleave the stealthy PEG shell）PubMed ID: 28489341FoldedExpand
- Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo AssessmentAuthorLei, Z;Chen, H;Zhang, H;Wang, Y;Meng, X;Wang, Z;Year2017JournalACS Appl Mater InterfacesApplicationenzymePubMed ID: 29190077FoldedExpand
- A coiled-coil masking domain for selective activation of therapeutic antibodiesAuthorTrang, VH;Zhang, X;Yumul, RC;Zeng, W;Stone, IJ;Wo, SW;Dominguez, MM;Cochran, JH;Simmons, JK;Ryan, MC;Lyon, RP;Senter, PD;Levengood, MR;Year2019JournalNat. Biotechnol.ApplicationenzymePubMed ID: 31133742FoldedExpand
- Peptide Microarray-Based Fluorescence Assay for Quantitatively Monitoring the Tumor-Associated Matrix Metalloproteinase-2 ActivityAuthorJian, M;Su, M;Gao, J;Wang, Z;Year2019JournalSensors and Actuators B: ChemicalApplicationenzymeFoldedExpand
- Alterations of gut microbiota contribute to the progression of unruptured intracranial aneurysmsAuthorLi, H;Xu, H;Li, Y;Jiang, Y;Hu, Y;Liu, T;Tian, X;Zhao, X;Zhu, Y;Wang, S;Zhang, C;Ge, J;Wang, X;Wen, H;Bai, C;Sun, Y;Song, L;Zhang, Y;Hui, R;Cai, J;Chen, J;Year2020JournalNat CommunPubMed ID: 32587239FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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