华生物/IL-4蛋白，人，重组/1/11846-HNAE
商品编号：
11846-HNAE
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IL-4 Protein, Human, Recombinant: Product Information
Purity
> 92 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL.
Protein Construction
A DNA sequence encoding the mature form of human IL4 isoform 1 (P05112-1) (His 25-Ser 153) was expressed and purified with an initial Met.
Accession#
P05112-1
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human IL4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15 kDa as predicted.
Formulation
Lyophilized from sterile 50mM Tris, 300mM NaCl, pH 7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL-4 Protein, Human, Recombinant: Images
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL.
IL-4 Protein, Human, Recombinant: Synonyms
BCGF-1 Protein, Human; BCGF1 Protein, Human; BSF-1 Protein, Human; BSF1 Protein, Human; IL-4 Protein, Human; Interleukin-4 Protein, Human
IL-4 Background Information
Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
interleukin 4
Related Pathways
Common G-Chain signaling pathway
References
- Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
- Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
- Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.
Human IL-4 Proteins
- IL-4 Protein, Human, Recombinant, BiotinylatedGMP-11846-HNAE-B
- IL-4 Protein, Human, RecombinantGMP-11846-HNAE
Mouse IL-4 Proteins
- IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag)51084-M08B
- IL-4 Protein, Mouse, Recombinant51084-MNAE
Canine IL-4 Proteins
- IL-4 Protein, Canine, Recombinant70021-DNAE
- Molecular cloning, recombinant expression and characterization of GMCSF from the rhesus monkey, Macaca mulattaAuthorTao, Z;Yang, H;Jia, D;Wan, L;Cheng, J;Lu, X;Year2013JournalDev. Comp. Immunol.Applicationcell-based (culture)PubMed ID: 23352624FoldedExpand
- IL-1β from M2 macrophages promotes migration and invasion of ESCC cells enhancing epithelial-mesenchymal transition and activating NF-κB signaling pathwayAuthorZhou, J;Zheng, S;Liu, T;Liu, Q;Chen, Y;Tan, D;Ma, R;Lu, X;Year2018JournalJ. Cell. Biochem.PubMed ID: 29737564FoldedExpand
- ROCK Inhibitor Y27632 Induced Morphological Shift and Enhanced Neurite Outgrowth-Promoting Property of Olfactory Ensheathing Cells via YAP-Dependent Up-Regulation of L1-CAMAuthorLi, Y;Huo, S;Fang, Y;Zou, T;Gu, X;Tao, Q;Xu, H;Year2018JournalFront. Cell. Neurosci.Applicationcell-basedPubMed ID: 30618636FoldedExpand
- Urotensin II, urotensin-related peptide, and their receptor in aortic valve stenosisAuthorKhan, K;Albanese, I;Yu, B;Shalal, Y;Al-Kindi, H;Alaws, H;Tardif, J;Gourgas, O;Cerutti, M;Schwertani, A;Year2019JournalThe Journal of Thoracic and Cardiovascular SurgeryApplicationcell-basedFoldedExpand
- Combination therapy with dendritic cell vaccine and programmed death ligand 1 immune checkpoint inhibitor for hepatocellular carcinoma in an orthotopic mouse modelAuthorTeng, C;Wang, T;Wu, T;Lin, J;Shih, F;Shyu, W;Jeng, L;Year2020JournalTher Adv Med OncolFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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