Sino Biological/BTK Protein, Human, Recombinant (His Tag)/1/10578-H08B
商品编号：
10578-H08B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
BTK Protein, Human, Recombinant (His Tag): Product Information
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
The specific activity was determined to be ＞50 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.
Protein Construction
A DNA sequence encoding the human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag.
Accession#
NP_000052.1
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The secreted recombinant human BTK consists of 670 amino acids and predicts a molecular mass of 77.8 KDa.
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0.Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
BTK Protein, Human, Recombinant (His Tag): Images
BTK Protein, Human, Recombinant (His Tag): Synonyms
AGMX1 Protein, Human; AT Protein, Human; ATK Protein, Human; BPK Protein, Human; IMD1 Protein, Human; PSCTK1 Protein, Human; XLA Protein, Human
BTK Background Information
Bruton"s tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton"s agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
Bruton agammaglobulinemia tyrosine kinase
Research Areas
- Cancer Drug Targets
Related Pathways
NF-kB (NFkB) Pathway
B Cell Receptor Signaling Pathway
References
- Hashimoto S, et al. (1996) Identification of Bruton"s tyrosine kinase (Btk) gene mutations and characterization of the derived proteins in 35 X-linked agammaglobulinemia families: a nationwide study of Btk deficiency in Japan. Blood. 88(2): 561-73.
- Ohta Y, et al. (1994) Genomic organization and structure of Bruton agammaglobulinemia tyrosine kinase: localization of mutations associated with varied clinical presentations and course in X chromosome-linked agammaglobulinemia. PNAS. 91(19): 9062-6.
- Smith C, et al. (1994) Expression of Bruton"s agammaglobulinemia tyrosine kinase gene, BTK, is selectively down-regulated in T lymphocytes and plasma cells. The Journal of Immunology. 152(2): 557-65.
- Chitosan/sulfobutylether-β-cyclodextrin nanoparticles for ibrutinib delivery: a potential nanoformulation of novel kinase inhibitorAuthorZhao, L;Tang, B;Tang, P;Sun, Q;Suo, Z;Zhang, M;Gan, N;Yang, H;Li, H;Year2019JournalJ Pharm SciApplicationenzymePubMed ID: 31606544FoldedExpand
- BTK operates a phospho-tyrosine switch to regulate NLRP3 inflammasome activityAuthorBittner, ZA;Liu, X;Dickhoefer, S;Kalbacher, H;Bosch, K;Year2019JournalbioRxivApplicationdot blot analysisFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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