Sino Biological/Argonaute-2 Protein, Human, Recombinant (His Tag)/1/11079-H07B
商品编号：
11079-H07B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Argonaute-2 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Human AGO2 (11079-H07B) can bind Let-7a RNA and cleave target RNA (21nt).
Protein Construction
A DNA sequence encoding the full length of human AGO2 (NP_036286.2) (Met 1-Ala 859) was expressed with a polyhistidine tag at the N-terminus.
Accession#
NP_036286.2
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human AGO2 consists of 877 amino acids and predicts a molecular mass of 99 kDa as estimated by SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4,10% glycerol,2mM DTTPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Argonaute-2 Protein, Human, Recombinant (His Tag): Images
Argonaute-2 Protein, Human, Recombinant (His Tag): Synonyms
Argonaute 2 Protein, Human; EIF2C2 Protein, Human; Q10 Protein, Human
Argonaute-2 Background Information
Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5"-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.
Full Name
argonaute RISC catalytic component 2
References
- O"Carroll D, et al. (2007) A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev. 21(16): 1999-2004.
- Pepper AS, et al. (2009) Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS One. 4(10): e7618.
- Lima WF, et al. (2009) Binding and cleavage specificities of human Argonaute2. J Biol Chem. 284(38): 26017-28.
- Adams BD, et al. (2009) Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150(1): 14-23.
- Salvatore V, et al. (2010) Bacterial expression of mouse argonaute 2 for functional and mutational studies. Int J Mol Sci. 11(2): 745-53.
- Wilson JA, et al. (2011) Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 85(5): 2342-50.
Mouse Argonaute-2 Proteins
- Argonaute-2 Protein, Mouse, Recombinant (His Tag)50683-M07B
- MicroRNA Directly Enhances Mitochondrial Translation during Muscle DifferentiationAuthorZhang, X;Zuo, X;Yang, B;Li, Z;Xue, Y;Zhou, Y;Huang, J;Zhao, X;Zhou, J;Yan, Y;Zhang, H;Guo, P;Sun, H;Guo, L;Zhang, Y;Fu, XD;Year2014JournalCellApplicationWBPubMed ID: 25083871FoldedExpand
- DICER-ARGONAUTE2 complex in continuous fluorogenic assays of RNA interference enzymesAuthorBernard, MA;Wang, L;Tachado, SD;Year2015JournalPLoS ONEApplicationenzymePubMed ID: 25793518FoldedExpand
- Preassembled Single-Stranded RNA-Argonaute Complexes: A Novel Method to Silence Genes in CryptosporidiumAuthorCastellanos-Gonzalez, A;Perry, N;Nava, S;White, AC;Year2015JournalJ. Infect. Dis.ApplicationbindingPubMed ID: 26656125FoldedExpand
- Cryptosporidium parvum Subtilisin-Like Serine Protease (SUB1) Is Crucial for Parasite Egress from Host CellsAuthorNava, S;White, AC;Castellanos-González, A;Year2019JournalInfect. Immun.ApplicationenzymePubMed ID: 30782859FoldedExpand
- Detection of MicroRNAs Released from ArgonautesAuthorMin, KW;Evans, JG;Won, EC;Yoon, JH;Year2020JournalMethods Mol. Biol.Applicationcomplex formationPubMed ID: 31889256FoldedExpand
- A well-designed Gold nanoparticle based fluorescence probe for assay Argonaute2 and Let-7a interaction in living cellsAuthorZhang, K;Fan, Z;Huang, Y;Xie, M;Zhao, J;Wang, J;Year2020JournalSensors and Actuators B: ChemicalFoldedExpand
- Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egressAuthorNava, S;Sadigova, A;Castellanos-Gonzalez, A;White, A;Year2020JournalMolecular and Biochemical ParasitologyApplicationassemble the RNA-silencer complexFoldedExpand
- Treatment of breast cancer with autophagy inhibitory microRNAs carried by AGO2-conjugated nanoparticlesAuthorUnal, O;Akkoc, Y;Kocak, M;Nalbat, E;Dogan-Ekici, AI;Yagci Acar, H;Gozuacik, D;Year2020JournalJ NanobiotechnologyApplicationRNA carrier nanoparticlePubMed ID: 32345308FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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