Sino Biological/IL2 Protein, Human, Recombinant/1/GMP-11848-HNAE
商品编号：
GMP-11848-HNAE
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IL2 Protein, Human, Recombinant: Product Information
Purity
> 95 % as determined by SDS-PAGE.
Endotoxin
< 1.0="" eu="" mg="" as="" determined="" by="" the="" lal="">
Activity
1. Measured in a cell proliferation assay using CTLL2. The ED50 for this effect is typically 0.5-3 ng/mL. The specific activity of recombinant human IL-2 is approximately 2×10E4 IU/μg.2. Using the Octet RED System, the affinity constant (Kd) of IL2 Protein, Human, Recombinant (Cat. GMP-11848-HNAE) bound Anti-IL2 Antibody (Cat. 11848-MM03) was 0.1 nM.
Protein Construction
A DNA sequence encoding the mature form of human IL2 (P60568-1) (Ala 21-Thr 153) was expressed and purified, with an initial Met.
Accession#
P60568-1
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human IL-2 consists of 134 amino acids and predicts a molecular mass of 15.5 kDa.
Formulation
Lyophilized froma 0.2μm filtered solution of Na2HPO4, NaH2PO4 and SDS.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL2 Protein, Human, Recombinant: Images
Measured in a cell proliferation assay using CTLL2. The ED50 for this effect is typically 0.5-3 ng/mL. The specific activity of recombinant human IL-2 is approximately 2×10E4 IU/μg.
Using the Octet RED System, the affinity constant (Kd) of IL2 Protein, Human, Recombinant (Cat. GMP-11848-HNAE) bound Anti-IL2 Antibody (Cat. 11848-MM03) was 0.1 nM.
IL2 Protein, Human, Recombinant: Synonyms
IL-2 Protein, Human; Interleukin-2 Protein, Human; lymphokine Protein, Human; TCGF Protein, Human
IL2 Background Information
Interleukin-2, also known as a T-cell growth factor, TCGF, Aldesleukin, and IL2, is a secreted protein that belongs to the IL-2 family. Interleukin-2 / IL-2 was the first interleukin molecule to be discovered. Interleukin-2 / IL-2 molecule was first purified to homogeneity by immunoaffinity chromatography by Kendall Smith and his team at Dartmouth Medical School. Interleukin-2 / IL-2 was also the first cytokine shown to mediate its effects via a specific IL-2 receptor, and it was also the first interleukin to be cloned and expressed from a complementary DNA (cDNA) library. Interleukin-2 / IL-2 was designated number 2 because Smith"s data at the time indicated that IL-1, produced by macrophages, facilitates IL-2 production by T lymphocytes (T cells).Interleukin-2 / IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 is normally produced by the body during an immune response. When environmental substances (molecules or microbes) gain access to the body, these substances (termed antigens) are recognized as foreign by antigen receptors that are expressed on the surface of lymphocytes. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2 / IL-2 and the expression of IL-2 receptors IL-2R. The IL-2 / IL-2R interaction then stimulates the growth, differentiation, and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2 / IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2 / IL-2 for clinical use is manufactured by Chiron Corporation with the brand name Proleukin. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2 / IL-2 in HIV therapy is ineffective.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
interleukin 2
Research Areas
- Neuroinflammation
Related Pathways
Common G-Chain signaling pathway
References
- Smith KA, et al.,1980, J. Exp. Med.151(6): 1551-6.
- Smith KA, et al.,1980, Nature. 287(5785): 853-5.
- Taniguchi T, et al.,1983, Nature. 302(5906): 305.
- Cantrell DA, et al.,1984, Science.224(4655): 1312-6.
- Smith KA, et al.,1988, Science.240(4856): 1169-76.
- Wang X. et al., 2005, Science 310:1159-63.
- Stauber D.J. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 2788-93.
Human IL2 Proteins
- IL2 Protein, Human, Recombinant, BiotinylatedGMP-11848-HNAE-B
- IL2 Protein, Human, Recombinant (L38M, L39S, C145A)11848-HNAY1
Mouse IL2 Proteins
- IL2 Protein, Mouse, Recombinant51061-MNAE
Rat IL2 Proteins
- IL2 Protein, Rat, Recombinant80075-RNAE
Canine IL2 Proteins
- IL2 Protein, Canine, Recombinant (147 Cys/Ser)70014-DNAE
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- TGFβ inhibition via CRISPR promotes the long-term efficacy of CAR-T cells against solid tumorsAuthorTang, N;Cheng, C;Zhang, X;Qiao, M;Li, N;Mu, W;Wei, XF;Han, W;Wang, H;Year2020JournalJCI InsightApplicationcell-basedPubMed ID: 31999649FoldedExpand
- Competition between PAF1 and MLL1/COMPASS confers the opposing function of LEDGF/p75 in HIV latency and proviral reactivationAuthorGao, R;Bao, J;Yan, H;Xie, L;Qin, W;Ning, H;Huang, S;Cheng, J;Zhi, R;Li, Z;Tucker, B;Chen, Y;Zhang, K;Wu, X;Liu, Z;Gao, X;Hu, D;Year2020JournalSci AdvApplicationcell culturePubMed ID: 32426500FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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