Sino-Biological/EGFR蛋白，人，重组（ECD，His-Tag）/1/10001-H08H
商品编号：
10001-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EGFR Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc (Cat: 10605-H01H) at 2 μg/ml (100 μl/well) can bind Human EGFR His (Cat: 10001-H08H), the EC50 of Human EGFR His is 100-500 ng/mL.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag.
Accession#
NP_005219
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Leu 25
Molecule Mass
The recombinant human EGF receptor consists of 630 amino acids and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 kDa protein in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EGFR Protein, Human, Recombinant (ECD, His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc (Cat: 10605-H01H) at 2 μg/ml (100 μl/well) can bind Human EGFR His (Cat: 10001-H08H), the EC50 of Human EGFR His is 100-500 ng/mL.
EGFR Protein, Human, Recombinant (ECD, His Tag): Synonyms
ERBB Protein, Human; ERBB1 Protein, Human; HER1 Protein, Human; mENA Protein, Human; NISBD2 Protein, Human; PIG61 Protein, Human
EGFR Background Information
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
epidermal growth factor receptor
Research Areas
- Cancer Drug Targets
- Receptor Tyrosine Kinases (RTKs)
Related Pathways
EGFR Signaling Pathway
Jak-Stat Signaling Pathway
References
- Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
- Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
- Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
Human EGFR Proteins
- EGFR Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated10001-H27H-B
- EGFR Protein, Human, Recombinant (His Tag)10001-H08S
- EGFR Protein, Human, Recombinant (aa 668-1210, His & GST Tag)10001-H20B2
- EGFR Protein, Human, Recombinant (ECD, His Tag), Biotinylated10001-H08H-B
- EGFR Protein, Human, Recombinant (Isoform vIII, His Tag)29662-H08B
- EGFR Protein, Human, Recombinant (Isoform vIII, His Tag), Biotinylated29662-H08B-B
- EGFR Protein, Human, Recombinant (Isoform Viii, hFc Tag)29662-H02B
- EGFR Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated29662-H27B-B
- EGFR Protein, Human, Recombinant (ECD, hFc Tag)10001-H02H
- EGFR Protein, Human, Recombinant (ECD, His Tag)10001-H08B
Mouse EGFR Proteins
- EGFR Protein, Mouse, Recombinant (hFc Tag)51091-M02H
- EGFR Protein, Mouse, Recombinant (His Tag)51091-M08H
Rat EGFR Proteins
- EGFR Protein, Rat, Recombinant (His Tag)80100-R08H
Cynomolgus EGFR Proteins
- EGFR Protein, Cynomolgus, Recombinant (His Tag)90285-C08B
- EGFR Protein, Cynomolgus, Recombinant (His Tag)90285-C08H
Rhesus EGFR Proteins
- EGFR Protein, Rhesus, Recombinant (ECD, His Tag)90317-K08B
- EGFR Protein, Rhesus, Recombinant (ECD, His Tag)90317-K08H
- EGFR Protein, Rhesus, Recombinant (ECD, hFc Tag)90317-K02H
Canine EGFR Proteins
- EGFR Protein, Canine, Recombinant (His Tag)70026-D08B
- Phage-derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGFAuthorZhang, J;Li, H;Wang, X;Qi, H;Miao, X;Zhang, T;Chen, G;Wang, M;Year2012JournalBiotechnol. Prog.Applicationbinding (negative control)PubMed ID: 22581629FoldedExpand
- Phage‐derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGFAuthorJ Zhang, H Li, X Wang, H Qi, X Miao…Year2012JournalBiotechnol. ProgApplicationnegative control in the test of the specificity of scFv antibodiesPubMed ID: 22581629FoldedExpand
- Erbin interacts with c-Cbl and promotes tumourigenesis and tumour growth in colorectal cancer by preventing c-Cbl-mediated ubiquitination and down-regulation of EGFRAuthorYao, S;Zheng, P;Wu, H;Song, LM;Ying, XF;Xing, C;Li, Y;Xiao, ZQ;Zhou, XN;Shen, T;Chen, L;Liu, YH;Lai, M;Mei, L;Gao, TM;Li, JM;Year2014JournalJ. Pathol.PubMed ID: 25521828FoldedExpand
- VHH characterization.Recombinant VHHs: Production, characterization and affinityAuthorChabrol, E;Stojko, J;Nicolas, A;Botzanowski, T;Fould, B;Antoine, M;Cianférani, S;Ferry, G;Boutin, J;Year2019JournalAnalytical BiochemistryFoldedExpand
- Enzymes Photo-Cross-Linked to Live Cell Receptors Retain Activity and EGFR Inhibition after Both Internalization and RecyclingAuthorRoy, S;Brasino, M;Beirne, JM;Harguindey, A;Chapnick, DA;Liu, X;Cha, JN;Goodwin, AP;Year2019JournalBioconjug. Chem.Applicationcell-basedPubMed ID: 31840981FoldedExpand
- Effects of viral promoters, the Woodchuck hepatitis post-transcriptional regulatory element, and weakened antibiotic resistance markers on transgene expression in Chinese hamster ovary cellsAuthorWang, W;Guo, X;Chen, S;Wang, T;Wang, F;Li, Q;Wang, Y;Year2020JournalProcess BiochemistryApplicationWB; ElisaFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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