Sino Biological/CD47 Protein, Human, Recombinant (ECD, hFc Tag)/1/12283-H02H
商品编号：
12283-H02H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CD47 Protein, Human, Recombinant (ECD, hFc Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His (Cat:11612-H08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml.2. Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His (Cat:50956-M08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.05-0.13 μg/ml.3. Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His (Cat:11828-H08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.58-1.34 μg/ml.
Protein Construction
A DNA sequence encoding the human CD47 (NP_942088.1) (Met1-Pro139) was expressed with the Fc region of human IgG1 at the C-terminus.
Accession#
NP_942088.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 19
Molecule Mass
The recombinant human CD47/Fc is a disulfide-linked homodimer. The reduced monomer comprises 362 amino acids and has a predicted molecular mass of 40.7 kDa. The apparent molecular mass of the protein is approximately 48-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD47 Protein, Human, Recombinant (ECD, hFc Tag): Images
1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His (Cat: 11612-H08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml.2. Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His (Cat: 50956-M08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.05-0.13 μg/ml.3. Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His (Cat: 11828-H08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.58-1.34 μg/ml.
CD47 Protein, Human, Recombinant (ECD, hFc Tag): Synonyms
IAP Protein, Human; MER6 Protein, Human; OA3 Protein, Human
CD47 Background Information
CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.
- Cancer Immunotherapy
- Co-inhibitory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
Full Name
CD47 molecule
Research Areas
- Cancer Drug Targets
References
- Brown EJ, et al. (2001) Integrin-associated protein (CD47) and its ligands. Trends Cell Biol. 11(3): 130-5.
- Oldenborg PA. (2004) Role of CD47 in erythroid cells and in autoimmunity. Leuk Lymphoma. 45(7): 1319-27.
- Kaczorowski DJ, et al. (2007) Targeting CD47: NO limit on therapeutic potential. Circ Res. 100(5): 602-3.
Human CD47 Proteins
- CD47 Protein, Human, Recombinant (ECD)12283-HCCH
- CD47 Protein, Human, Recombinant (ECD, His Tag)12283-H08H
- CD47 Protein, Human, Recombinant (His & AVI Tag), Biotinylated12283-H27H-B
- CD47 Protein, Human, Recombinant (ECD, His Tag)12283-H08B
- CD47 Protein, Human, Recombinant, Biotinylated12283-HCCH-B
Mouse CD47 Proteins
- CD47 Protein, Mouse, Recombinant (His Tag)57231-M08H
- CD47 Protein, Mouse, Recombinant (ECD)57231-MNAH
- CD47 Protein, Mouse, Recombinant (ECD, hFc Tag)57231-M02H
- CD47 Protein, Mouse, Recombinant (rFc Tag)57231-M31H
Rat CD47 Proteins
- CD47 Protein, Rat, Recombinant (His Tag)80305-R08H
- CD47 Protein, Rat, Recombinant (hFc Tag)80305-R02H
Cynomolgus CD47 Proteins
- CD47 Protein, Cynomolgus, Recombinant (hFc Tag)90869-C02H
Cynomolgus, Rhesus CD47 Proteins
- CD47 Protein, Cynomolgus, Rhesus, Recombinant (ECD, His Tag)90869-C08H
- CD40 Enhances Type I Interferon Responses Downstream of CD47 Blockade, Bridging Innate and Adaptive ImmunityAuthorde Silva, S;Fromm, G;Shuptrine, CW;Johannes, K;Patel, A;Yoo, KJ;Huang, K;Schreiber, TH;Year2019JournalCancer Immunol ResPubMed ID: 31852716FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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