Sino Biological/Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag)/1/10973-H08C
商品编号：
10973-H08C
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag): Product Information
Purity
> 75 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the pro form of human von Willebrand factor (AAB59458.1) (Met1-Lys2813) was expressed with a C-terminal polyhistidine tag.
Accession#
AAB59458.1
Expressed Host
CHO Stable Cells
Species
Human
Predicted N Terminal
Ala 23
Molecule Mass
The secreted recombinant human vWF consists of 2802 amino acids after removal of the signal peptide and has a predicted molecular mass of 308 kDa. Purified rhvWf exists as both the pro form with the pro peptide (307 kDa) and the mature form (226 kDa), which migrates as doublets with apparent molecular mass of 260 and 350 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag): Images
Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag): Synonyms
F8VWF Protein, Human; Von Willebrand Factor Protein, Human; VWD Protein, Human
Von Willebrand Factor/vWF Background Information
Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde"s syndrome, possibly hemolytic-uremic syndrome and so on.
Full Name
von Willebrand factor
References
- Sadler JE. (1998) Biochemistry and genetics of von Willebrand factor. Annu Rev Biochem, 67: 395-424.
- Batlle J, et al. (2009) Von Willebrand factor/factor VIII concentrates in the treatment of von Willebrand disease. Blood Coagul Fibrinolysis. 20(2):89-100.
- Sadler JE. (2009) von Willebrand factor assembly and secretion. J Thromb Haemost. 7 Suppl 1:24-7.
- Auton M, et al. (2010) The mechanism of VWF-mediated platelet GPIbalpha binding. Biophys J. 99(4):1192-201.
- Bowen DJ. (2010) Sugar targets VWF for the chop. Blood. 115(13):2565.
- Lpez JA, et al. (2010) VWF self-association: more bands for the buck. Blood. 116(19):3693-4.
- Novel antibodies against GPIbα inhibit pulmonary metastasis by affecting vWF-GPIbα interactionAuthorQi, Y;Chen, W;Liang, X;Xu, K;Gu, X;Wu, F;Fan, X;Ren, S;Liu, J;Zhang, J;Li, R;Liu, J;Liang, X;Year2018JournalJ Hematol OncolApplicationbindingPubMed ID: 30223883FoldedExpand
- Migfilin supports hemostasis and thrombosis through regulating platelet αIIbβ3 outside-in signalingAuthorZhou, Y;Hu, M;Chen, X;Wang, S;Li, J;Sa, L;Li, L;Huang, J;Cheng, H;Hu, H;Year2019JournalHaematologicaApplicationcell-basedPubMed ID: 31879325FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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