Sino Biological/NGF Protein, Human, Recombinant/1/11050-HNAC
商品编号：
11050-HNAC
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
NGF Protein, Human, Recombinant: Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.
Protein Construction
A DNA sequence encoding the mature form of human β-NGF (NP_002497.2) (Ser 122-Arg 239) was expressed.
Accession#
NP_002497.2
Expressed Host
CHO Stable Cells
Species
Human
Predicted N Terminal
Ser 122
Molecule Mass
The mature recombinant human β-NGF consists of 118 amino acids and has a predicted molecular mass of 13.2 kDa. β-NGF exists as a non-disulfide linked homodimer in solution.
Formulation
Lyophilized from sterile 40mM His, 40mM Arg, 150mM NaCl, pH 5.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
NGF Protein, Human, Recombinant: Images
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.
NGF Protein, Human, Recombinant: Synonyms
Beta-NGF Protein, Human; HSAN5 Protein, Human; NGFB Protein, Human
NGF Background Information
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
Full Name
nerve growth factor (beta polypeptide)
Related Pathways
Death Receptor Signaling
References
- Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
- Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson"s disease. J Mol Neurosci. 35(2): 161-9.
- Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.
Human NGF Proteins
- NGF Protein, Human, Recombinant11050-HNAE
- NGF Protein, Human, Recombinant11050-HNAH
Mouse NGF Proteins
- NGF Protein, Mouse, Recombinant50385-MNAC
- β-Nerve growth factor is a major component of alpaca seminal plasma and induces ovulation in female alpacasAuthorKershaw-Young, CM;Druart, X;Vaughan, J;Maxwell, WM;Year2012JournalReprod. Fertil. Dev.PubMed ID: 22951217FoldedExpand
- Clinical significance of nerve growth factor and tropomyosin-receptor-kinase signaling pathway in intrahepatic cholangiocarcinomaAuthorYang, XQ;Xu, YF;Guo, S;Liu, Y;Ning, SL;Lu, XF;Yang, H;Chen, YX;Year2014JournalWorld J. Gastroenterol.Applicationcell-basedPubMed ID: 24744599FoldedExpand
- Conformational Analysis of Large and Highly Disulfide-Stabilized Proteins by Integrating Online Electrochemical Reduction into an Optimized H/D Exchange Mass Spectrometry WorkflowAuthorTrabjerg, E;Jakobsen, RU;Mysling, S;Christensen, S;Jørgensen, TJ;Rand, KD;Year2015JournalAnal. Chem.PubMed ID: 26249042FoldedExpand
- Investigating the conformational response of the Sortilin receptor upon binding endogenous peptide- and protein ligands by HDX-MSAuthorTrabjerg, E;Abu-Asad, N;Wan, Z;Kartberg, F;Christensen, S;Rand, K;Year2018JournalbioRxivApplicationbindingFoldedExpand
- Histone deacetylase 6 inhibition restores autophagic flux to promote functional recovery after spinal cord injuryAuthorZheng, Z;Zhou, Y;Ye, L;Lu, Q;Zhang, K;Zhang, J;Xie, L;Wu, Y;Xu, K;Zhang, H;Xiao, J;Year2019JournalExp. Neurol.Applicationcell-basedPubMed ID: 31794745FoldedExpand
- Nerve growth factor-induced migration in oral and salivary gland tumour cells utilizes the PI3K/Akt signalling pathway: is there a link to perineural invasion?AuthorAlkhadar, H;Macluskey, M;White, S;Ellis, I;Year2019JournalJ. Oral Pathol. Med.Applicationcell-basedPubMed ID: 31782565FoldedExpand
- TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stressAuthorZhou, K;Zheng, Z;Li, Y;Han, W;Zhang, J;Mao, Y;Chen, H;Zhang, W;Liu, M;Xie, L;Zhang, H;Xu, H;Xiao, J;Year2020JournalTheranosticsApplicationcell-basedPubMed ID: 32802192FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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