Sino Biological/HGF Protein, Human, Recombinant/1/10463-HNAS
商品编号：
10463-HNAS
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HGF Protein, Human, Recombinant: Product Information
Purity
> 93 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation.The ED50 for this effect is typically 2-10 ng/mL.
Protein Construction
A DNA sequence encoding the human HGF (NP_000592.3) precursor (Met 1-Ser 728) was expressed and purified.
Accession#
NP_000592.3
Expressed Host
CHO Stable Cells
Species
Human
Predicted N Terminal
Gln 32 (α chain) & Val 495 (β chain)
Molecule Mass
The secreted recombinant human HGF consists of 697 amino acids after cleavage of the signal peptide and has a predicted molecular mass of 79.7 kDa. The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. As a result of glycosylation, it migrates with the apparent molecular mass of 90, 60 and 34 kDa corresponding to the single chain, α chain and β chain respectively in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, 1mM Arg, .5% tween2, pH 7.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
HGF Protein, Human, Recombinant: Images
Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 2-10 ng/mL.
HGF Protein, Human, Recombinant: Synonyms
DFNB39 Protein, Human; F-TCF Protein, Human; F-TCFB Protein, Human; Hepatocyte Growth Factor Protein, Human; HGFB Protein, Human; HPTA Protein, Human; SF Protein, Human
HGF Background Information
Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain, and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. The hepatocyte growth factor is secreted by mesenchymal cells as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility and matrix invasion give it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
hepatocyte growth factor (hepapoietin A; scatter factor)
Research Areas
- Cancer Drug Targets
References
- Naldini L, et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78.
- Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
- Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
- Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.
Mouse HGF Proteins
- HGF Protein, Mouse, Recombinant (His Tag)50038-M08H
- HGF Protein, Mouse, Recombinant50038-MNAH
Rat HGF Proteins
- HGF Protein, Rat, Recombinant80429-RNAH
Cynomolgus HGF Proteins
- HGF Protein, Cynomolgus, Recombinant90286-CNAH
Canine HGF Proteins
- HGF Protein, Canine, Recombinant70063-DNAH
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- Motor Neuron-Skeletal Muscle Co Culture Model: A Potential Novel in Vitro and Computaional Platform to Investigate Cancer CachexiaAuthorAl Samid, M;Al-Shanti, N;Odeh, M;Year2018Journal2018 1st International Conference on Cancer Care Informatics (CCI)Applicationcell-basedFoldedExpand
- A Short Isoform of Coagulation Factor XII mRNA Is Expressed by Neurons in the Human BrainAuthorZamolodchikov, D;Bai, Y;Tang, Y;McWhirter, JR;Macdonald, LE;Alessandri-Haber, N;Year2019JournalNeuroscienceApplicationenzymePubMed ID: 31181367FoldedExpand
- Simplified in vitro engineering of functional mammalian neuromuscular junctions between embryonic rat motor neurons and immortalised human skeletal muscle cellsAuthorSaini, J;Year2019JournalThesisApplicationcell-basedFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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