Sino Biological/EpCAM Protein, Human, Recombinant (ECD, His Tag)/1/10694-H08H
商品编号：
10694-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EpCAM Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 96 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-His coated plates (1.25μg/mL, 100μL/well), approximately >40% will adhere specifically after 30 minutes at 37℃.
Protein Construction
A DNA sequence encoding the extracellular domain (Met1-Lys265) of human EpCAM (NP_002345.1) was fused with a polyhistidine tag at the C-terminus.
Accession#
NP_002345.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 24
Molecule Mass
The recombinant human EpCAM consists of 253 amino acids after removal of the signal peptide and has a calculated molecular mass of 28.84 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human EpCAM is approximately 36.5 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EpCAM Protein, Human, Recombinant (ECD, His Tag): Images
EpCAM Protein, Human, Recombinant (ECD, His Tag): Synonyms
DIAR5 Protein, Human; EGP-2 Protein, Human; EGP314 Protein, Human; EGP40 Protein, Human; ESA Protein, Human; HNPCC8 Protein, Human; KS1/4 Protein, Human; KSA Protein, Human; M4S1 Protein, Human; MIC18 Protein, Human; MK-1 Protein, Human; TACSTD1 Protein, Human; TROP-1 Protein, Human; TROP1 Protein, Human
EpCAM Background Information
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
epithelial cell adhesion molecule
References
- Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
- Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
- Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
- Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
Human EpCAM Proteins
- EpCAM Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated10694-H27H-B
- EpCAM Protein, Human, Recombinant (ECD, hFc Tag)10694-H02H
- EpCAM Protein, Human, Recombinant (hFc Tag), Biotinylated10694-H02H-B
- EpCAM Protein, Human, Recombinant (His Tag), Biotinylated10694-H08H-B
- EpCAM Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated, HPLC-verifiedHPLC-10694-H27H-B
- EpCAM Protein, Human, Recombinant (ECD, hFc & AVI Tag), Biotinylated10694-H41H-B
Mouse EpCAM Proteins
- EpCAM Protein, Mouse, Recombinant (His Tag)50591-M08H
Rat EpCAM Proteins
- EpCAM Protein, Rat, Recombinant (His Tag)80306-R08H
- EpCAM Protein, Rat, Recombinant (hFc Tag)80306-R02H
Cynomolgus EpCAM Proteins
- EpCAM Protein, Cynomolgus, Recombinant (His Tag)90299-C08H
Cynomolgus, Rhesus EpCAM Proteins
- EpCAM Protein, Cynomolgus, Rhesus, Recombinant90299-CCCH
- EpCAM Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)90299-C02H
- Fluorescence-guided tumor detection with a novel anti-EpCAM targeted antibody fragment: Preclinical validationAuthorBoogerd, L;Boonstra, M;Prevoo, H;Handgraaf, H;Kuppen, P;van de Velde, C;Fish, A;Cordfunke, R;Valentijn, A;Terwisscha van Scheltinga, A;MacDonald, G;Cizeau, J;Premsukh, A;Vinkenburg van Slooten, M;Burggraaf, J;Sier, C;Vahrmeijer, A;Year2019JournalSurgical OncologyApplicationSPRFoldedExpand
- In silico design and validation of high-affinity RNA aptamers targeting epithelial cellular adhesion molecule dimersAuthorBell, DR;Weber, JK;Yin, W;Huynh, T;Duan, W;Zhou, R;Year2020JournalProc. Natl. Acad. Sci. U.S.A.ApplicationITCPubMed ID: 32234785FoldedExpand
- HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassaysAuthorBae, Y;Jang, D;Eom, S;Park, T;Kang, S;Year2020JournalSensors and Actuators B: ChemicalApplicationelisaFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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