华生物/TNF-α蛋白，人，重组/1/10602-HNAE
商品编号：
10602-HNAE
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TNF-alpha Protein, Human, Recombinant: Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
1. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.2. Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Protein Construction
A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
Accession#
NP_000585.2
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.4 kDa which is also estimated by SDS-PAGE.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TNF-alpha Protein, Human, Recombinant: Images
Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio;Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
TNF-alpha Protein, Human, Recombinant: Synonyms
DIF Protein, Human; TNF-alpha Protein, Human; TNFA Protein, Human; TNFSF2 Protein, Human
TNF-alpha Background Information
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & Antibody
TNF-alpha Protein & Antibody- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
tumor necrosis factor
Research Areas
- Cancer Drug Targets
- Neuroinflammation
Related Pathways
Death Receptor Signaling
NF-kB (NFkB) Pathway
IL17 signaling pathway
References
- Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
- Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
Human TNF-alpha Proteins
- TNF-alpha Protein, Human, Recombinant (hFc Tag)10602-H01H
- TNF-alpha Protein, Human, Recombinant, Biotinylated10602-HNAE-B
- TNF-alpha Protein, Human, RecombinantGMP-10602-HNAE
Mouse TNF-alpha Proteins
- TNF-alpha Protein, Mouse, Recombinant (ECD)50349-MNAE
- TNF-alpha Protein, Mouse, Recombinant, Biotinylated50349-MNAE-B
Rat TNF-alpha Proteins
- TNF-alpha Protein, Rat, Recombinant80045-RNAE
Cynomolgus TNF-alpha Proteins
- TNF-alpha Protein, Cynomolgus, Recombinant90330-CNAE
- TNF-alpha Protein, Cynomolgus, Recombinant, Biotinylated90330-CNAE-B
Canine TNF-alpha Proteins
- TNF-alpha Protein, Canine, Recombinant70003-DNAE
Ferret TNF-alpha Proteins
- TNF-alpha Protein, Ferret, Recombinant60002-FNAE
Cynomolgus, Rhesus TNF-alpha Proteins
- TNF-alpha Protein, Cynomolgus, Rhesus, Recombinant90018-CNAE
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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