Sino Biological/CD13蛋白，人，重组（603-Met/Ile，His-Tag）/1/10051-H08H
商品编号：
10051-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CD13 Protein, Human, Recombinant (603 Met/Ile, His Tag): Product Information
Purity
> 97 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >2,500 pmoles/min/μg.
Protein Construction
A DNA sequence encoding the metalloprotease domain (Lys 69-Lys 967，603 Met/Ile) of the mature form of human ANPEP (NP_001141.2) was expressed with N-terminal signal peptide and C-terminal polyhistidine tag.
Accession#
NP_001141.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Lys 69
Molecule Mass
The recombinant human ANPEP protein consists of 910 amino acids and has a predicted molecular mass of 104.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhANPEP is approximately 135-140 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.3Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD13 Protein, Human, Recombinant (603 Met/Ile, His Tag): Images
CD13 Protein, Human, Recombinant (603 Met/Ile, His Tag): Synonyms
APN Protein, Human; CD13 Protein, Human; GP150 Protein, Human; LAP1 Protein, Human; P150 Protein, Human; PEPN Protein, Human
CD13 Background Information
Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.
Full Name
alanyl (membrane) aminopeptidase
References
- Pasqualini R, et al. (2000) Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis.Cancer Res. 60(3): 722-7.
- Mabjeesh SJ, et al. (2005) Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide. J Dairy Sci. 88(6): 2055-64.
- Dunkel B, et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131(1-2): 25-32.
Mouse CD13 Proteins
- CD13 Protein, Mouse, Recombinant (His Tag)50001-M08H
- Recombinant IFN-α2a-NGR exhibits higher inhibitory function on tumor neovessels formation compared with IFN-α2a in vivo and in vitroAuthorW Li, Q Hao, L He, J Meng, M Li, X Xue, C Zhang, H Li…Year2014JournalCytotechnologyApplicationELISAPubMed ID: 24897998FoldedExpand
- The pro-inflammatory cytokine 14-3-3ε is a ligand of CD13 in cartilageAuthorNefla, M;Sudre, L;Denat, G;Priam, S;Andre-Leroux, G;Berenbaum, F;Jacques, C;Year2015JournalJ. Cell. Sci.ApplicationSPRFoldedExpand
- NGR-tagged nano-gold: A new CD13-selective carrier for cytokine delivery to tumorsAuthorCurnis, F;Fiocchi, M;Sacchi, A;Gori, A;Gasparri, A;Corti, A;Year2016JournalNano ResApplicationenzyme and bindingFoldedExpand
- Rational Design of Peptide Ligands Based on Knob–Socket Protein Packing Model Using CD13 as a Prototype ReceptorAuthorUddin, M;Li, X;Joo, H;Tsai, J;Wrischnik, L;Jasti, B;Year2019JournalACS OmegaApplicationinhibition assayFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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