Sino Biological/TLR4 Protein, Human, Recombinant (ECD, His Tag)/1/10146-H08B
商品编号：
10146-H08B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TLR4 Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 87 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human TLR4 (Met1-Lys631) (O00206-1) was expressed with a C-terminal polyhistidine tag.
Accession#
O00206-1
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Glu 24
Molecule Mass
The secreted recombinant human TLR4 consists of 619 amino acids and predicts a molecular mass of 70.5 KDa. The apparent molecular mass of the protein is approximately 68 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile 20mM PB, 500mM NaCl, pH 6.0, 10% glycerolPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TLR4 Protein, Human, Recombinant (ECD, His Tag): Images
TLR4 Protein, Human, Recombinant (ECD, His Tag): Synonyms
ARMD10 Protein, Human; CD284 Protein, Human; TLR-4 Protein, Human; TLR4 Protein, Human; TOLL Protein, Human
TLR4 Background Information
TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).
Full Name
toll-like receptor 4
Research Areas
- Neuroinflammation
Related Pathways
NF-kB (NFkB) Pathway
References
- Re, Fabio, et al. (2002) Monomeric recombinant MD-2 binds toll-like receptor 4 tightly and confers lipopolysaccharide responsiveness. J Biol Chem. 277(26):23427-32.
- Shimazu, R, et al. (1999) MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4. J Exp Med. 189(11):1777-82.
- Blanco, A M, et al. (2005) Involvement of TLR4/type I IL-1 receptor signaling in the induction of inflammatory mediators and cell death induced by ethanol in cultured astrocytes. Journal of immunology. 175(10):6893-9.
- Catalpol restores LPS-elicited rat microcirculation disorder by regulation of a network of signaling involving inhibition of TLR-4 and SrcAuthorZhang, YP;Pan, CS;Yan, L;Liu, YY;Hu, BH;Chang, X;Li, Q;Huang, DD;Sun, HY;Fu, G;Sun, K;Fan, JY;Han, JY;Year2016JournalAm. J. Physiol. Gastrointest. Liver Physiol.ApplicationSPRFoldedExpand
- Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4AuthorYang, J;Qin, N;Zhang, H;Yang, R;Xiang, B;Wei, Q;Year2016JournalSci RepApplicationbindingPubMed ID: 27090571FoldedExpand
- Towards automation in biologics production via Raman micro-spectroscopy, laser-induced forward cell transfer and surface-enhanced Raman spectroscopyAuthorJaeckle, E;Brauchle, E;Nottrodt, N;Wehner, M;Lensing, R;Gillner, A;Schenke-Layland, K;Bach, M;Burger-Kentischer, A;Year2020JournalJ. Biotechnol.ApplicationWBPubMed ID: 32898625FoldedExpand
- Arctigenin protects against depression by inhibiting microglial activation and neuroinflammation via HMGB1/TLR4/NF‐κB and TNF‐α/TNFR1/NF‐κB pathwaysAuthorXu, X;Piao, HN;Aosai, F;Zeng, XY;Cheng, JH;Year2020JournalBritish Journal of PharmacologyFoldedExpand
If you couldn"t find the specific citation, you can:
- Find All Citations
- Submit a CitationGet a Publication Reward
- Leave Us a Message
E.coli protein expression service
Mammalian transient protein expression service
Baculovirus-insect expression service
Stable cell line development service (CHO / HEK293)
品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
联络我们
