Sino Biological/IL3RA Protein, Human, Recombinant (ECD, His Tag)/1/10518-H08H
商品编号：
10518-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IL3RA Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind biotinylated human IL3RA with a linear range of 0.32-1.6 μg/ml.
Protein Construction
A DNA sequence encoding the human IL3RA (NP_002174.1) extracellular domain (Met1-Arg305) was expressed with a C-terminal polyhistidine tag.
Accession#
NP_002174.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Thr 19
Molecule Mass
The secreted recombinant human IL3RA comprises 298 amino acids and has a predicted molecular mass of 34.5 kDa. As a result of glycosylation, the apparent molecular mass of rh IL3RA is approximately 50-60 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL3RA Protein, Human, Recombinant (ECD, His Tag): Images
IL3RA Protein, Human, Recombinant (ECD, His Tag): Synonyms
CD123 Protein, Human; hIL-3Ra Protein, Human; IL3R Protein, Human; IL3RAY Protein, Human; IL3RX Protein, Human; IL3RY Protein, Human
IL3RA Background Information
Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and/or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.
Full Name
interleukin 3 receptor, alpha (low affinity)
Related Pathways
AKT Signaling Pathway
Jak-Stat Signaling Pathway
Autophagy Pathway
References
- Del Giudice I, et al. (2004) The diagnostic value of CD123 in B-cell disorders with hairy or villous lymphocytes. Haematologica. 89(3): 303-8.
- Du X, et al. (2007) New immunotoxins targeting CD123, a stem cell antigen on acute myeloid leukemia cells. J Immunother. 30(6): 607-13.
- Yue LZ, et al. (2010) Expression of CD123 and CD114 on the bone marrow cells of patients with myelodysplastic syndrome. Chin Med J (Engl). 123(15): 2034-2037.
Human IL3RA Proteins
- IL3RA Protein, Human, Recombinant (hFc Tag)10518-H02H
- IL3RA Protein, Human, Recombinant (His & hFc Tag)10518-H03H
Mouse IL3RA Proteins
- IL3RA Protein, Mouse, Recombinant (ECD, His Tag)50810-M08H
- IL3RA Protein, Mouse, Recombinant (ECD, hFc Tag)50810-M02H
Canine IL3RA Proteins
- IL3RA Protein, Canine, Recombinant (His Tag)70077-D08H
- Optimization of human NK cell manufacturing: Fully-automated separation, improved <i>ex vivo </i>expansion using IL-21 with autologous feeder cells and generation of anti-CD123-CAR-expressing effector cellsAuthorKlöß, S;Oberschmidt, O;Morgan, M;Dahlke, J;Arseniev, L;Huppert, V;Granzin, M;Gardlowski, T;Matthies, N;Soltenborn, S;Schambach, A;Koehl, U;Year2017JournalHum. Gene Ther.ApplicationCAR-T cell detectionPubMed ID: 28810809FoldedExpand
- Programmed cell death protein 1 activation preferentially inhibits CD28.CAR-T cellsAuthorZolov, SN;Rietberg, SP;Bonifant, CL;Year2018JournalCytotherapyApplicationFCM (CAR-T)PubMed ID: 30309710FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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