Sino Biological/PD-1 Protein, Human, Recombinant (ECD, His Tag)/1/10377-H08H
商品编号：
10377-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PD-1 Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 μg/ml (100 μl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 μg/ml.2. Labeled biotin to PD-1 Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-1 Protein, Human, Recombinant (His Tag), Biotinylated(Cat. 10377-H08H-B) bound to Keytruda was 4.7 nM.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Gln 167) of human PD1 (NP_005009.2) was expressed with a C-terminal polyhistidine tag.
Accession#
NP_005009.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Leu 25
Molecule Mass
The recombinant mature human PD1 consists of 154 amino acids and has a calculated molecular mass of 17.4 kDa. As a result of glycosylation, human PD1 migrates as an approximately 41.1 kDa protein in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 50mM MOPS, 500mM NaCl, pH 7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PD-1 Protein, Human, Recombinant (ECD, His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 μg/ml (100 μl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 μg/ml.
Labeled biotin to PD-1 Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-1 Protein, Human, Recombinant (His Tag), Biotinylated(Cat. 10377-H08H-B) bound to Keytruda was 4.7 nM.
PD-1 Protein, Human, Recombinant (ECD, His Tag): Synonyms
CD279 Protein, Human; hPD-1 Protein, Human; hPD-l Protein, Human; hSLE1 Protein, Human; PD-1 Protein, Human; PD1 Protein, Human; SLEB2 Protein, Human
PD-1 Background Information
Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
- Cancer Immunotherapy
- Co-inhibitory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Blockade: Blocking Antibodi
- Immune Checkpoint Blockade: PD1 / PDCD1 / CD2
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: IHC Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Proteins
- Immune Checkpoint Targets
- Immunotherapy
- PD1 / PDCD1 / CD279 Immune Checkpoint
- PD1 / PDCD1 / CD279 Immune Checkpoint Antibod
- PD1 / PDCD1 / CD279 Immune Checkpoint Protein
- Targeted Therapy
Full Name
programmed cell death 1
Research Areas
- ITIM/ITAM Immunoreceptors and Related Molecules
- Cancer Drug Targets
References
- James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.
- Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.
- del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.
- Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
Human PD-1 Proteins
- PD-1 Protein, Human, Recombinant (His Tag), Biotinylated10377-H08H-B
- PD-1 Protein, Human, Recombinant (ECD, hFc Tag)10377-H02H
- PD-1 Protein, Human, Recombinant (ECD, His & hFc Tag), Biotinylated10377-H03H-B
- PD-1 Protein, Human, Recombinant (His Tag), HPLC-verifiedHPLC-10377-H08H
- PD-1 Protein, Human, Recombinant (ECD, His & hFc Tag)10377-H03H
- PD-1 Protein, Human, Recombinant (ECD, mFc Tag)10377-H05H
- PD-1 Protein, Human, Recombinant (ECD, hFc Tag)HPLC-10377-H02H
Mouse PD-1 Proteins
- PD-1 Protein, Mouse, Recombinant (ECD, His Tag)50124-M08H
- PD-1 Protein, Mouse, Recombinant (ECD, His & hFc Tag)50124-M03H
- PD-1 Protein, Mouse, Recombinant (ECD, hFc Tag)50124-M02H
- PD-1 Protein, Mouse, Recombinant (His Tag), Biotinylated50124-M08H-B
Rat PD-1 Proteins
- PD-1 Protein, Rat, Recombinant (ECD, His Tag)80448-R08H
- PD-1 Protein, Rat, Recombinant (hFc Tag)80448-R02H
Cynomolgus PD-1 Proteins
- PD-1 Protein, Cynomolgus, Recombinant (hFc & AVI Tag), Biotinylated90311-C41H-B
- PD-1 Protein, Cynomolgus, Recombinant (His Tag)90311-C08H
- PD-1 Protein, Cynomolgus, Recombinant (hFc Tag)90311-C02H
Canine PD-1 Proteins
- PD-1 Protein, Canine, Recombinant (His Tag)70109-D08H
- PD-1 Protein, Canine, Recombinant (His & AVI Tag), Biotinylated70109-D27H-B
- PD-1 Protein, Canine, Recombinant (hFc Tag)70109-D02H
Rhesus PD-1 Proteins
- PD-1 Protein, Rhesus, Recombinant (His Tag)90305-K08H
- PD-1 Protein, Rhesus, Recombinant (hFc Tag)90305-K02H
- Design, isolation and evaluation of the binding efficiency of a DNA aptamer against interleukin 2 receptor alpha, in vitroAuthorShahdordizadeh, M;Taghdisi, SM;Sankian, M;Ramezani, M;Abnous, K;Year2017JournalInt. Immunopharmacol.PubMed ID: 29055191FoldedExpand
- Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1AuthorLi, D;Xu, J;Wang, Z;Gong, Z;Liu, J;Zheng, Y;Li, J;Li, J;Year2017JournalmAbsApplicationbindingPubMed ID: 28300475FoldedExpand
- Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35AuthorZhao, M;Wang, J;Luo, M;Luo, H;Zhao, M;Han, L;Zhang, M;Yang, H;Xie, Y;Jiang, H;Feng, L;Lu, H;Zhu, J;Year2018JournalAppl. Microbiol. Biotechnol.ApplicationSPRPubMed ID: 29789882FoldedExpand
- Enzyme linked immunosorbent assay for the quantification of nivolumab and pembrolizumab in human serum and cerebrospinal fluidAuthorPluim, D;Ros, W;van Bussel, MTJ;Brandsma, D;Beijnen, JH;Schellens, JHM;Year2018JournalJ Pharm Biomed AnalApplicationELISA (PK)PubMed ID: 30368118FoldedExpand
- Enhanced therapeutic efficacy of a novel oncolytic herpes simplex virus type 2 encoding an antibody against programmed cell death 1AuthorZhu, Y;Hu, X;Feng, L;Yang, Z;Zhou, L;Duan, X;Cheng, S;Zhang, W;Liu, B;Zhang, K;Year2019JournalMolecular Therapy - OncolyticsApplicationWBFoldedExpand
- PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 PathwaysAuthorZhao, Y;Lee, CK;Lin, CH;Gassen, RB;Xu, X;Huang, Z;Xiao, C;Bonorino, C;Lu, LF;Bui, JD;Hui, E;Year2019JournalImmunityApplicationcell staining and bindingPubMed ID: 31757674FoldedExpand
- Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitorsAuthorFan, Z;Tian, Y;Chen, Z;Liu, L;Zhou, Q;He, J;Coleman, J;Dong, C;Li, N;Huang, J;Xu, C;Zhang, Z;Gao, S;Zhou, P;Ding, K;Chen, L;Year2020JournalEMBO Mol MedApplicationElisaPubMed ID: 32391629FoldedExpand
- Development and Evaluation of Novel Aptamers Specific for Human PD1 Using Hybrid Systematic Evolution of Ligands by Exponential Enrichment ApproachAuthorKhedri, M;Abnous, K;Rafatpanah, H;Nabavinia, MS;Taghdisi, SM;Ramezani, M;Year2020JournalImmunol. Invest.ApplicationSELEX; FACS; ElisaPubMed ID: 32429721FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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