Sino Biological/PD-L2 Protein, Mouse, Recombinant (His Tag)/1/50804-M08H
商品编号：
50804-M08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PD-L2 Protein, Mouse, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse PD-L2-his at 10 μg/mL (100 μl/well) can bind mouse PD1-Fc. The EC50 of mouse PD1-Fc is 1.63 μg/mL.
Protein Construction
A DNA sequence encoding the extracellular domain of mouse PDCD1LG2 (Q9WUL5) (Met 1-Arg 219) was expressed, with a C-terminal polyhistidine tag.
Accession#
Q9WUL5
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Leu 20
Molecule Mass
The secreted recombinant mouse PDCD1LG2 comprises 211 amino acids and has a calculated molecular mass of 24 kDa. As a result of glycosylation, the apparent molecular mass of rmPDCD1LG2 is approximately 35-45 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PD-L2 Protein, Mouse, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized mouse PD-L2-his at 10 μg/mL (100 μl/well) can bind mouse PD1-Fc. The EC50 of mouse PD1-Fc is 1.63 μg/mL.
PD-L2 Protein, Mouse, Recombinant (His Tag): Synonyms
B7-DC Protein, Mouse; Btdc Protein, Mouse; F730015O22Rik Protein, Mouse; PD-L2 Protein, Mouse
PD-L2 Background Information
Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.
- Cancer Immunotherapy
- Co-inhibitory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: FCM Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Proteins
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
Full Name
programmed cell death 1 ligand 2
Research Areas
- Cancer Drug Targets
References
- Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8.
- Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30.
- Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.
Human PD-L2 Proteins
- PD-L2 Protein, Human, Recombinant (ECD, His Tag)10292-H08H
- PD-L2 Protein, Human, Recombinant (hFc Tag), HPLC-verifiedHPLC-10292-H02H
- PD-L2 Protein, Human, Recombinant (His & hFc Tag)10292-H03H
- PD-L2 Protein, Human, Recombinant (His Tag), Biotinylated10292-H08H-B
- PD-L2 Protein, Human, Recombinant (ECD, mFc Tag)10292-H38H
- PD-L2 Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated10292-H27H-B
- PD-L2 Protein, Human, Recombinant (hFc Tag)10292-H02H
Mouse PD-L2 Proteins
- PD-L2 Protein, Mouse, Recombinant (His Tag), Biotinylated50804-M08H-B
- PD-L2 Protein, Mouse, Recombinant (hFc Tag)50804-M02H
Rat PD-L2 Proteins
- PD-L2 Protein, Rat, Recombinant (His Tag)80454-R08H
- PD-L2 Protein, Rat, Recombinant (hFc Tag)80454-R02H
Cynomolgus PD-L2 Proteins
- PD-L2 Protein, Cynomolgus, Recombinant (His Tag)90249-C08H
- PD-L2 Protein, Cynomolgus, Recombinant (hFc Tag)90249-C02H
- PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 PathwaysAuthorZhao, Y;Lee, CK;Lin, CH;Gassen, RB;Xu, X;Huang, Z;Xiao, C;Bonorino, C;Lu, LF;Bui, JD;Hui, E;Year2019JournalImmunityPubMed ID: 31757674FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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