Sino Biological/AGO1 Protein, Human, Recombinant (His Tag)/1/11225-H07B
商品编号：
11225-H07B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AGO1 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the full length of human AGO1 (NP_036331.1) (Met 1-Ala 857) was expressed, with a polyhistidine tag at the N-terminus.
Accession#
NP_036331.1
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
His
Molecule Mass
The recombinant human AGO1 consists of 875 amino acids and predicts a molecular mass of 99.5 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Gly, 0.5 PMSF, 0.5mM EDTA, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
AGO1 Protein, Human, Recombinant (His Tag): Images
AGO1 Protein, Human, Recombinant (His Tag): Synonyms
EIF2C Protein, Human; EIF2C1 Protein, Human; GERP95 Protein, Human; Q99 Protein, Human
AGO1 Background Information
Protein argonaute-1, also known aseukaryotic translation initiation factor 2C 1, EIF2C1, and AGO1, is a member of the argonaute family and agosubfamily. Protein argonaute-1 in humans is encoded by the EIF2C1 gene. Thisgene is located on chromosome 1 in a cluster of closely related family membersincluding argonaute 3, and argonaute 4. This genomic region is frequently lostin human cancers such as Wilms tumors, neuroblastoma, and carcinomas of thebreast, liver, and colon. The human EIF2C1 gene is ubiquitously expressed atlow to medium levels. Differential polyadenylation and splicing result in acomplex transcriptional pattern.EIF2C1 protein contains onePAZdomain and onePiwi domain. It is required for RNA-mediated gene silencing(RNAi) and transcriptional gene silencing (TGS) of promoter regions which arecomplementary to bound short antigene RNAs (agRNAs). EIF2C1 binds to short RNAssuch as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and repressesthe translation of mRNAs which are complementary to them.
Full Name
argonaute RISC catalytic component 1
References
- Koesters R., et al.,(1999), Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization, localization to chromosomal bands 1p34-p35, and expression. Genomics 61:210-218.
- Gregory S.G., et al., (2006), The DNA sequence and biological annotation of human chromosome 1.Nature 441:315-321.
- Motamedi M.R., et al.,(2004), Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs. Cell. 119:789-802.
- Quantitative functions of Argonaute proteins in mammalian developmentAuthorWang, D;Zhang, Z;O"Loughlin, E;Lee, T;Houel, S;O"Carroll, D;Tarakhovsky, A;Ahn, NG;Yi, R;Year2012JournalGenes Dev.PubMed ID: 22474261FoldedExpand
- DNA Tetrahedron Based Biosensor for Argonaute2 Assay in Single Cells and Human Immunodeficiency Virus Type-1 Related Ribonuclease H Detection in VitroAuthorZhang, K;Huang, W;Huang, Y;Li, H;Wang, K;Zhu, X;Xie, M;Year2019JournalAnal. Chem.Applicationbinding (DNA)PubMed ID: 31050888FoldedExpand
- A well-designed Gold nanoparticle based fluorescence probe for assay Argonaute2 and Let-7a interaction in living cellsAuthorZhang, K;Fan, Z;Huang, Y;Xie, M;Zhao, J;Wang, J;Year2020JournalSensors and Actuators B: ChemicalApplicationbindingFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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