Sino Biological/TRAIL/TNFSF10 Protein, Rhesus, Recombinant/1/90098-CNAE
商品编号：
90098-CNAE
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TRAIL/TNFSF10 Protein, Rhesus, Recombinant: Product Information
Purity
> 98 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
1. Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-2 ng/mL.2. Measured by its binding ability in a functional ELISA. Immobilized Rhesus TNFSF10 at 10 μg/ml (100 μl/well) can bind Rhesus TNFRSF10D-Fc (Cat:90100-C02H), The EC50 of Rhesus TNFRSF10D-Fc (Cat:90100-C02H) is 7.8-18.1 ng/ml.
Protein Construction
A DNA sequence encoding the rhesus TNFSF10 (NP_001252963.1) (Val114-Gly281) was expressed and purified with an initial Met.
Accession#
NP_001252963.1
Expressed Host
E. coli
Species
Rhesus
Predicted N Terminal
Met
Molecule Mass
The recombinant rhesus TNFSF10 consists of 169 amino acids and has a calculated molecular mass of 19.6 kDa.
Formulation
Lyophilized from sterile PBSPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TRAIL/TNFSF10 Protein, Rhesus, Recombinant: Images
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-2 ng/mL.
TRAIL/TNFSF10 Background Information
Tumor necrosis factor ligand superfamily member 10 (TNFSF10), also known as TNF-related apoptosis-inducing ligand (TRAIL), Apo-2 ligand, and CD253, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TNFSF10 / Apo-2L / CD253 functions as a ligand that induces the process of cell death called apoptosis. TNFSF10 / TRAIL shows homology to other members of the tumor necrosis factor superfamily. As one member of the cluster of differentiation system, TNFSF10 / CD253 is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion TNFSF10 / Apo-2L / CD253 / TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A / TRAILR1, TNFRSF10B / TRAILR2, TNFRSF10C / TRAILR3, TNFRSF10D / TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TNFSF10 / TRAIL may be modulated by binding to the decoy receptors TNFRSF10C / TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8 / JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
tumor necrosis factor (ligand) superfamily, member 10
Research Areas
- Cancer Drug Targets
Related Pathways
Death Receptor Signaling
p53 Pathway
References
- Song C, et al. (2005) TRAIL (CD253), a new member of the TNF superfamily. J Biol Regul Homeost Agents. 19(1-2): 73-7.
- Kuribayashi K, et al. (2008) TNFSF10 (TRAIL), a p53 target gene that mediates p53-dependent cell death. Cancer Biol Ther. 7(12): 2034-8.
- Wiley SR, et al. (1995) Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity. 3(6): 673-82.
Human TRAIL/TNFSF10 Proteins
- TRAIL/TNFSF10 Protein, Human, Recombinant10409-HNAE
Mouse TRAIL/TNFSF10 Proteins
- TRAIL/TNFSF10 Protein, Mouse, Recombinant (aa 118-291, His Tag)50166-M07E
- Preclinical studies of a death receptor 5 fusion protein that ameliorates acute liver failureAuthorChen, Q;Wang, P;Zhang, Q;Xia, M;Zhang, G;Li, J;Shen, E;Chen, YH;Wan, X;Year2019JournalJ. Mol. Med.PubMed ID: 31230087FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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