Sino Biological/EphB4 Protein, Mouse, Recombinant (His Tag)/1/50582-M08H
商品编号：
50582-M08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EphB4 Protein, Mouse, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse EphB4 at 2 μg/ml (100 μl/well) can bind Rat EphrinB2. The EC50 of Rat EphrinB2 is 20-100 ng/mL
Protein Construction
A DNA sequence encoding the mouse EPHB4 (P54761-1) extracellular domain (Met 1-Ala 539) was fused with a polyhistidine tag at the C-terminus.
Accession#
P54761-1
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Leu 16
Molecule Mass
The secreted recombinant mouse EPHB4 comprises 535 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, the apparent molecular mass of rm EPHB4/His monomer is approximately 70 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EphB4 Protein, Mouse, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized mouse EphB4 at 2 μg/ml (100 μl/well) can bind Rat EphrinB2, The EC50 of Rat EphrinB2 is 20-100 ng/mL.
EphB4 Protein, Mouse, Recombinant (His Tag): Synonyms
AI042935 Protein, Mouse; Htk Protein, Mouse; MDK2 Protein, Mouse; Myk1 Protein, Mouse; Tyro11 Protein, Mouse
EphB4 Background Information
Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.
Full Name
EPH receptor B4
Related Pathways
Actin Dynamics Signaling Pathway
G Protein-coupled Receptors Signaling
MAPK-Erk Pathway
Autophagy Pathway
References
- Davalos V, et al. (2006) EPHB4 and survival of colorectal cancer patients. Cancer Res. 66(18): 8943-8.
- Zhao C, et al. (2006) Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab. 4(2): 111-21.
- Kertesz N, et al. (2006) The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth. Blood. 107(6): 2330-8.
- Noren NK, et al. (2007) Paradoxes of the EphB4 receptor in cancer. Cancer Res. 67(9): 3994-7.
- Taylor AC, et al. (2007) EphB4 expression along adult rat microvascular networks: EphB4 is more than a venous specific marker. Microcirculation. 14(3): 253-67.
Human EphB4 Proteins
- EphB4 Protein, Human, Recombinant (hFc Tag)10235-H02H
- EphB4 Protein, Human, Recombinant (aa 563-987, His & GST Tag)10235-H20B1
- EphB4 Protein, Human, Recombinant (His Tag), Biotinylated10235-H08H-B
- EphB4 Protein, Human, Recombinant (His Tag)10235-H08H
- EphB4 Protein, Human, Recombinant10235-HCCH
Mouse EphB4 Proteins
- EphB4 Protein, Mouse, Recombinant (hFc Tag)50582-M02H
- Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBDAuthorGrandi, A;Zini, I;Palese, S;Giorgio, C;Tognolini, M;Marchesani, F;Bruno, S;Flammini, L;Cantoni, A;Castelli, R;Lodola, A;Fusari, A;Barocelli, E;Bertoni, S;Year2019JournalFront. Pharmacol.ApplicationELISAPubMed ID: 31297055FoldedExpand
If you couldn"t find the specific citation, you can:
- Find All Citations
- Submit a CitationGet a Publication Reward
- Leave Us a Message
E.coli protein expression service
Mammalian transient protein expression service
Baculovirus-insect expression service
Stable cell line development service (CHO / HEK293)
品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
联络我们
