Sino Biological/Neuropilin-1 Protein, Human, Recombinant (ECD, V179A, hFc Tag)/1/10011-H02H
商品编号：
10011-H02H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Neuropilin-1 Protein, Human, Recombinant (ECD, V179A, hFc Tag): Product Information
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to bind with recombinant human VEGF165 in a functional ELISA.
Protein Construction
A DNA sequence encoding the human NRP1 isoform b (NP_001019799.1, with natural variant Val 179 Ala) (Met1-Lys644) was expressed with the Fc region of human IgG1 at the C-terminus.
Accession#
NP_001019799.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Phe 22
Molecule Mass
The recombinant human NRP1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 861 amino acids and has a calculated molecular mass of 96.5 kDa. As a result of glycosylation, rh NRP1/Fc monomer migrates as an approximately 107.4 kDa protein in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Neuropilin-1 Protein, Human, Recombinant (ECD, V179A, hFc Tag): Images
Neuropilin-1 Protein, Human, Recombinant (ECD, V179A, hFc Tag): Synonyms
BDCA4 Protein, Human; CD304 Protein, Human; Neuropilin-1 Protein, Human; NP1 Protein, Human; NRP Protein, Human; VEGF165R Protein, Human
Neuropilin-1 Background Information
Neuropilin is a type I transmembrane protein and the molecular mass is 120 kDa. Two homologs, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins, and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.
Full Name
neuropilin 1
References
- Nakamura F, et al. (2002) Structural and functional relation of neuropilins. Adv Exp Med Biol. 515: 55-69.
- Romeo PH, et al. (2002) Neuropilin-1 in the immune system. Adv Exp Med Biol. 515: 49-54.
- Klagsbrun M, et al. (2002) The role of neuropilin in vascular and tumor biology. Adv Exp Med Biol. 515: 33-48.
- Staton CA, et al. (2007) Neuropilins in physiological and pathological angiogenesis. J Pathol. 212(3): 237-48.
- Bagri A, et al. (2009) Neuropilins in tumor biology. Clin Cancer Res. 15(6): 1860-4.
Human Neuropilin-1 Proteins
- Neuropilin-1 Protein, Human, Recombinant (His Tag)10011-H08H
- Neuropilin-1 Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated10011-H27H-B
Mouse Neuropilin-1 Proteins
- Neuropilin-1 Protein, Mouse, Recombinant (ECD, His Tag)50509-M08H
- Neuropilin-1 Protein, Mouse, Recombinant (ECD, mFc Tag)50509-M38H
Cynomolgus Neuropilin-1 Proteins
- Neuropilin-1 Protein, Cynomolgus, Recombinant (His Tag)90297-C08H
- Neuropilin-1 Protein, Cynomolgus, Recombinant90297-CCCH
- Neuropilin-1 Protein, Cynomolgus, Recombinant (hFc Tag)90297-C02H
- Evidence for Pro-angiogenic Functions of VEGF-AxAuthorXin, H;Zhong, C;Nudleman, E;Ferrara, N;Year2016JournalCellApplicationbindingPubMed ID: 27662093FoldedExpand
- Neuropilin-1 drives tumor-specific uptake of chlorotoxinAuthorMcGonigle, S;Majumder, U;Kolber-Simonds, D;Wu, J;Hart, A;Noland, T;TenDyke, K;Custar, D;Li, D;Du, H;Postema, MHD;Lai, WG;Twine, NC;Woodall-Jappe, M;Nomoto, K;Year2019JournalCell Commun. SignalApplicationOctetPubMed ID: 31208428FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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