Sino Biological/DPP4/CD26 Protein, Human, Recombinant/1/10688-HNCH
商品编号：
10688-HNCH
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
DPP4/CD26 Protein, Human, Recombinant: Product Information
Purity
> 70 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 2,500 pmoles/min/μg.2. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V31B) was 20 nM.3. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V05B) was 50 nM.4. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 40069-V08B) was 33 nM.5. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike-His (aa 1-760) (Cat: 40021-V08H) was 12 nM.
Protein Construction
The native mature form of human DPPIV (NP_001926.2) extracellular domain (Asn 29-Pro 766) was expressed and purified.
Accession#
NP_001926.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Asn 29
Molecule Mass
The recombinant human DPPIV exists as the mature active form, and consists of 738 amino acids with a predicted molecular mass of 85.4 kDa. The apparent molecular mass of the glycosylated rhDPPIV is approximately 95 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
DPP4/CD26 Protein, Human, Recombinant: Images
DPP4/CD26 Protein, Human, Recombinant: Synonyms
ADABP Protein, Human; ADCP2 Protein, Human; CD26 Protein, Human; DPPIV Protein, Human; TP103 Protein, Human
DPP4/CD26 Background Information
Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.
Full Name
dipeptidylpeptidase 4
Research Areas
- Serine Proteases and Regulators
References
- Doupis J, et al. (2008) DPP4 inhibitors: a new approach in diabetes treatment. Adv Ther. 25(7): 627-43.
- Havre PA, et al. (2008) The role of CD26/dipeptidyl peptidase IV in cancer. Front Biosci. 13: 1634-45.
- De Chiara L, et al. (2009) Soluble CD26 levels and its association to epidemiologic parameters in a sample population. Dis Markers. 7(6): 311-6.
- Matteucci E, et al. (2009) Dipeptidyl peptidase-4 (CD26): knowing the function before inhibiting the enzyme. Curr Med Chem. 16(23): 2943-51.
Human DPP4/CD26 Proteins
- DPP4/CD26 Protein, Human, Recombinant (hFc Tag)10688-H01H
- DPP4/CD26 Protein, Human, Recombinant (His Tag)10688-H08H
Mouse DPP4/CD26 Proteins
- DPP4/CD26 Protein, Mouse, Recombinant (hFc Tag)50718-M01H
- DPP4/CD26 Protein, Mouse, Recombinant (His Tag)50718-M08H
Cynomolgus DPP4/CD26 Proteins
- DPP4/CD26 Protein, Cynomolgus, Recombinant (ECD, His Tag)90180-C08H
- DPP4/CD26 Protein, Cynomolgus, Recombinant90180-CNCH
- DPP4/CD26 Protein, Cynomolgus, Recombinant (hFc Tag)90180-C01H
- Antidiabetic Stilbenes from Peony Seeds with PTP1B, α-Glucosidase, and DPPIV Inhibitory ActivitiesAuthorZhang, CC;Geng, CA;Huang, XY;Zhang, XM;Chen, JJ;Year2019JournalJ. Agric. Food Chem.ApplicationenzymePubMed ID: 31180676FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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