Sino Biological/PTP1B Protein, Human, Recombinant (His Tag)/1/10304-H07E
商品编号：
10304-H07E
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PTP1B Protein, Human, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate, R&D Systems, Catalog # ES006.The specific activity is > 15 nmoles/min/μg.
Protein Construction
A DNA sequence encoding the human PTPN1 (NP_002818.1) (Glu 2-Asn 321) was expressed, with a polyhistide tag at the N-terminus.
Accession#
NP_002818.1
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human PTPN1 consisting of 327 amino acids and has a calculated molecular mass of 38.1 kDa.
Formulation
Supplied as sterile PBS, pH 7.4Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PTP1B Protein, Human, Recombinant (His Tag): Images
PTP1B Protein, Human, Recombinant (His Tag): Synonyms
PTP1B Protein, Human; PTPN1 Protein, Human
PTP1B Background Information
PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.
Full Name
protein tyrosine phosphatase, non-receptor type 1
Research Areas
- Phosphatases and Regulators
References
- Frangioni JV, et al. (1992) The nontransmembrane tyrosine phosphatase PTP-1B localizes to the endoplasmic reticulum via its 35 amino acid C-terminal sequence. Cell. 68(3):545-60.
- Zhu S, et al. (2007) PTP1B contributes to the oncogenic properties of colon cancer cells through Src activation. Cancer Res. 67(21):10129-37.
- Aoki N, et al. (2000) A cytosolic protein-tyrosine phosphatase PTP1B specifically dephosphorylates and deactivates prolactin-activated STAT5a and STAT5b. J Biol Chem. 275(50):39718-26.
- Stuible M, et al. (2008) PTP1B regulates cortactin tyrosine phosphorylation by targeting Tyr446. J Biol Chem. 283(23):15740-6.
- Preparation of Yellow-Green-Emissive Carbon Dots and Their Application in Constructing a Fluorescent Turn-On Nanoprobe for Imaging of Selenol in Living Cells.AuthorWang, Q;Zhang, S;Zhong, Y;Yang, XF;Li, Z;Li, H;Year2017JournalAnal. ChemApplicationassay validationPubMed ID: 28208245FoldedExpand
- Virtual Screening of Novel and Selective Inhibitors of Protein Tyrosine Phosphatase 1B over T-Cell Protein Tyrosine Phosphatase Using a Bidentate Inhibition StrategyAuthorChen, X;Gan, Q;Feng, C;Liu, X;Zhang, Q;Year2018JournalJ Chem Inf ModelApplicationenzymePubMed ID: 29608303FoldedExpand
- Virtual Screening of Protein Tyrosine Phosphatase 1B Inhibitors Based on Natural ProductsAuthorQian, Z;Qiang, G;Xia, L;Xi, C;Chang-Gen, F;Year2018JournalProgress in Biochemistry and BiophysicsApplicationenzymeFoldedExpand
- Antidiabetic Stilbenes from Peony Seeds with PTP1B, α-Glucosidase, and DPPIV Inhibitory ActivitiesAuthorZhang, CC;Geng, CA;Huang, XY;Zhang, XM;Chen, JJ;Year2019JournalJ. Agric. Food Chem.PubMed ID: 31180676FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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