Sino Biological/CD73 Protein, Human, Recombinant (His Tag)/1/10904-H08H
商品编号：
10904-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CD73 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) (Cat. 10904-H08H) bound MEDI9447 was 28 nM.2. Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP). The specific activity is > 15,000 pmoles/min/μg.
Protein Construction
A DNA sequence encoding the human NT5E (NP_002517.1) (Met1-Lys547) was expressed fused with a polyhistidine tag at the C-terminus.
Accession#
NP_002517.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Trp 27
Molecule Mass
The secreted recombinant human NT5E consists of 532 amino acids with the predicted molecular mass of 59.2 kDa.
Formulation
Lyophilized from sterile 20mM Tris, 120 mM NaCl, 4 mM CaCl2, 20 % glycerol, pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD73 Protein, Human, Recombinant (His Tag): Images
Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) (Cat. 10904-H08H) bound MEDI9447 was 28 nM.
CD73 Protein, Human, Recombinant (His Tag): Synonyms
CALJA Protein, Human; CD73 Protein, Human; E5NT Protein, Human; eN Protein, Human; eNT Protein, Human; NT Protein, Human; NT5 Protein, Human; NTE Protein, Human
CD73 Background Information
5"-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5"-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
Full Name
5"-nucleotidase, ecto (CD73)
Research Areas
- Phosphatases and Regulators
References
- Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
- Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
- Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
- Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
Human CD73 Proteins
- CD73 Protein, Human, Recombinant (His Tag), Biotinylated10904-H08H-B
- CD73 Protein, Human, Recombinant (His Tag), HPLC-verifiedHPLC-10904-H08H
- CD73 Protein, Human, Recombinant (hFc Tag)10904-H02H
Mouse CD73 Proteins
- CD73 Protein, Mouse, Recombinant (His Tag)50231-M08H
Rat CD73 Proteins
- CD73 Protein, Rat, Recombinant (His Tag)80335-R08H
Cynomolgus CD73 Proteins
- CD73 Protein, Cynomolgus, Recombinant (His Tag)90192-C08H
- Adenosine Receptor Agonists And CD73 Effects On Avm Cell Calcification MarkersAuthorKoliopoulos, T;Year2019JournalThesisApplicationcell-basedPubMed ID: 31214999FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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