Sino Biological/PLK1 Protein, Human, Recombinant (His Tag)/1/10676-H07B
商品编号：
10676-H07B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PLK1 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
The specific activity was determined to be ＞3 nmol/min/mg using casein as substrate.
Protein Construction
A DNA sequence encoding the human PLK1 (NP_005021.2) (Met 1-Ser 603) was expressed, with a polyhistidine tag at the N-terminus.
Accession#
NP_005021.2
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
His
Molecule Mass
The recombinant human PLK1 consists of 621 amino acids and predicts a molecular mass of 70.5 kDa. It migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 50mM Tris, 100mM NaCl, pH 7.4, 0.5mM EDTA, 0.5mM EGTA, 0.5mM PMSF, 25% glycerolPlease contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PLK1 Protein, Human, Recombinant (His Tag): Images
PLK1 Protein, Human, Recombinant (His Tag): Synonyms
PLK Protein, Human; STPK13 Protein, Human
PLK1 Background Information
Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well-characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.
Full Name
polo-like kinase 1
References
- Lee KS, et al. (2008) Self-regulated mechanism of Plk1 localization to kinetochores: lessons from the Plk1-PBIP1 interaction. Cell Div. 3: 4.
- Zhou T, et al. (2003) A role for Plk1 phosphorylation of NudC in cytokinesis. Dev Cell. 5 (1): 127-38.
- Lee M, et al. (2004) Phosphorylation of BRCA2 by the Polo-like kinase Plk1 is regulated by DNA damage and mitotic progression. Oncogene. 23 (4): 865-72.
Mouse PLK1 Proteins
- PLK1 Protein, Mouse, Recombinant (His Tag)50624-M07B
- Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathwayAuthorMa, X;Wang, L;Huang, ;Li, Y;Yang, D;Li, T;Li, F;Sun, L;Wei, H;He, K;Yu, F;Zhao, D;Hu, L;Xing, S;Liu, Z;Li, K;Guo, J;Yang, Z;Pan, X;Li, A;Shi, Y;Wang, J;Gao, P;Zhang, H;Year2017JournalNat CommunApplicationenzymePubMed ID: 29138396FoldedExpand
- Polo-Like Kinase 1 phosphorylates and stabilizes KLF4 to promote tumorigenesis in nasopharyngeal carcinomaAuthorMai, J;Zhong, ZY;Guo, GF;Chen, XX;Xiang, YQ;Li, X;Zhang, HL;Chen, YH;Xu, XL;Wu, RY;Yu, Y;Li, ZL;Peng, XD;Huang, Y;Zhou, LH;Feng, GK;Guo, X;Deng, R;Zhu, XF;Year2019JournalTheranosticsApplicationenzymePubMed ID: 31281496FoldedExpand
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E.coli protein expression service
Mammalian transient protein expression service
Baculovirus-insect expression service
Stable cell line development service (CHO / HEK293)
品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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