Sino Biological/E-Cadherin Protein, Human, Recombinant (ECD, His Tag)/1/10204-H08H
商品编号：
10204-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
E-Cadherin Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells.When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), approximately 20%-40% of cells will adhere specifically after 90 minutes at 37 ℃.
Protein Construction
A DNA sequence encoding the extracellular domain of human E-Cad precusor (NP_004351.1) (Met 1-Ile 707) was fused with a C-terminal polyhistidine tag.
Accession#
NP_004351.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 23 & Asp 155
Molecule Mass
The recombinant pro form of human E-Cad consists of 696 amino acids and has a calculated molecular mass of 77 kDa. As a result of glycosylation, the apparent molecular mass of rh E-Cad is approximately 90.6 kDa corresponding to the mature form and pro form respectively in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
E-Cadherin Protein, Human, Recombinant (ECD, His Tag): Images
E-Cadherin Protein, Human, Recombinant (ECD, His Tag): Synonyms
Arc-1 Protein, Human; CD324 Protein, Human; CDH1 Protein, Human; CDHE Protein, Human; E-cad Protein, Human; E-Cadherin Protein, Human; ECAD Protein, Human; LCAM Protein, Human; UVO Protein, Human
E-Cadherin Background Information
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
Full Name
cadherin 1, type 1, E-cadherin (epithelial)
Research Areas
- Hemangioblast Markers
- Tumor Suppressors
Related Pathways
Canonical Wnt Pathway
EGFR Signaling Pathway
References
- Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
- Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
- Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
Human E-Cadherin Proteins
- E-Cadherin Protein, Human, Recombinant (ECD, hFc Tag)10204-H02H
- E-Cadherin Protein, Human, Recombinant (His Tag)10204-H08H1
- E-Cadherin Protein, Human, Recombinant (hFc Tag), Biotinylated10204-H02H-B
- E-Cadherin Protein, Human, Recombinant (ECD, His Tag)10204-H08B
Mouse E-Cadherin Proteins
- E-Cadherin Protein, Mouse, Recombinant (His Tag)50671-M08H
Rat E-Cadherin Proteins
- E-Cadherin Protein, Rat, Recombinant (His Tag)80274-R08H
- E-Cadherin Protein, Rat, Recombinant (hFc Tag)80274-R02H
- The effect of adherens junction components on keratinocyte adhesion in vitro: potential implications for sealing the skin-implant interface of intraosseous transcutaneous amputation prosthesesAuthorCJ Pendegrass, B Tucker, S Patel…Year2012JournalJ Biomed Mater Res Part AApplicationE-cadherin adsorptionPubMed ID: 22815157FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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