Sino Biological/NGF Protein, Mouse, Recombinant/1/50385-MNAC
商品编号：
50385-MNAC
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
NGF Protein, Mouse, Recombinant: Product Information
Purity
> 96 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 1-8ng/mL.
Protein Construction
A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus.
Accession#
NP_001106168.1
Expressed Host
CHO Stable Cells
Species
Mouse
Predicted N Terminal
Met
Molecule Mass
The recombinant mouse NGF consists of 121 amino acids and has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
NGF Protein, Mouse, Recombinant: Images
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 1-8ng/mL.
NGF Protein, Mouse, Recombinant: Synonyms
Ngfb Protein, Mouse
NGF Background Information
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
Full Name
nerve growth factor (beta polypeptide)
Related Pathways
Death Receptor Signaling
References
- Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
- Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson"s disease. J Mol Neurosci. 35(2): 161-9.
- Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.
Human NGF Proteins
- NGF Protein, Human, Recombinant11050-HNAE
- NGF Protein, Human, Recombinant11050-HNAH
- NGF Protein, Human, Recombinant11050-HNAC
- Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture systemAuthorYuan, Q;Sun, L;Yu, H;An, C;Year2017JournalBiosci. Biotechnol. Biochem.Applicationcell based (culture)PubMed ID: 28394221FoldedExpand
- Biocompatible Electroactive Tetra(aniline)-Conjugated Peptide Nanofibers for Neural DifferentiationAuthorArioz, I;Erol, O;Bakan, G;Dikecoglu, FB;Topal, AE;Urel, M;Dana, A;Tekinay, AB;Guler, MO;Year2017JournalACS Appl Mater InterfacesApplicationcell-basedPubMed ID: 29232108FoldedExpand
- Irisin regulates the expression of BDNF and glycometabolism in diabetic ratsAuthorHuang, L;Yan, S;Luo, L;Yang, L;Year2018JournalMol Med RepApplicationN/aPubMed ID: 30569121FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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