Sino Biological/Glypican 3 Protein, Human, Recombinant (His Tag)/1/10088-H08H
商品编号：
10088-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Glypican 3 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 87 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized human GPC3 at 5 μg/ml (100 μl/well) can bind human bFGF / FGF2 with a linear range of 0.156-2.5 ng/ml.
Protein Construction
A DNA sequence encoding the human GPC3 (P51654-1) (Met1-His559) was expressed with a C-terminal polyhistidine tag.
Accession#
P51654-1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 25 & Ser 359
Molecule Mass
The recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 62 kDa, Cleaved partially by a furin-like convertase to N and C-terminal fragment with predicted molecular mass of 38 KDa and 24 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Glypican 3 Protein, Human, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized human GPC3 at 5 μg/ml (100 μl/well) can bind human bFGF / FGF2 with a linear ranger of 0.156-2.5 ng/ml.
Glypican 3 Protein, Human, Recombinant (His Tag): Synonyms
DGSX Protein, Human; Glypican 3 Protein, Human; GTR2-2 Protein, Human; MXR7 Protein, Human; OCI-5 Protein, Human; SDYS Protein, Human; SGB Protein, Human; SGBS Protein, Human; SGBS1 Protein, Human
Glypican 3 Background Information
Glypican-3, also known as Intestinal protein OCI-5, GPC3, and OCI5, is a member of the glypican family. It belongs to the glypican family and is highly expressed in the lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue-dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. Studies have shown that GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceed both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. The role of GPC3 in melanomas is still controversial. Thus, Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
glypican 3
Research Areas
- Cancer Drug Targets
References
- Kandil DH, et al. (2009) Glypican-3: a novel diagnostic marker for hepatocellular carcinoma and more. Adv Anat Pathol. 16(2): 125-9.
- Maeda D, et al. (2009) Glypican-3 expression in clear cell adenocarcinoma of the ovary. Mod Pathol. 22(6): 824-32.
Human Glypican 3 Proteins
- Glypican 3 Protein, Human, Recombinant (His Tag), Biotinylated10088-H08H-B
- Glypican 3 Protein, Human, Recombinant (495S/A, mFc Tag)10088-H38H
- Glypican 3 Protein, Human, Recombinant (495S/A, hFc Tag)10088-H02H2
Mouse Glypican 3 Proteins
- Glypican 3 Protein, Mouse, Recombinant (His Tag)50989-M08B
- A fluorescence based immunoassay for galectin-4 using gold nanoclusters and a composite consisting of glucose oxidase and a metal-organic frameworkAuthorZhang, X;Zeng, Y;Zheng, A;Cai, Z;Huang, A;Zeng, J;Liu, X;Liu, J;Year2017JournalMicrochimica ActaApplicationassay validationFoldedExpand
- Elevated GPC3 level promotes cell proliferation in liver cancerAuthorWang, S;Chen, N;Chen, Y;Sun, L;Li, L;Liu, H;Year2018JournalOncol LettApplicationcell-basedPubMed ID: 29963171FoldedExpand
- Immunization with glypican-3 nanovaccine containing TLR7 agonist prevents the development of carcinogen-induced precancerous hepatic lesions to cancer in a murine modelAuthorChen, K;Wu, Z;Zang, M;Wang, C;Wang, Y;Wang, D;Ma, Y;Qu, C;Year2018JournalAm J Transl ResApplicationcell-based (BMDC induction)PubMed ID: 30018715FoldedExpand
- CXCR2-modified CAR-T cells have enhanced trafficking ability that improves treatment of hepatocellular carcinomaAuthorLiu, G;Rui, W;Zheng, H;Huang, D;Yu, F;Zhang, Y;Dong, J;Zhao, X;Lin, X;Year2020JournalEur. J. Immunol.ApplicationFACSPubMed ID: 31981231FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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