Sino Biological/c-Abl/ABL1 Protein, Human, Recombinant (GST Tag)/1/11199-H09B
商品编号：
11199-H09B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
c-Abl/ABL1 Protein, Human, Recombinant (GST Tag): Product Information
Purity
> 75 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
The specific activity was determined to be 240 nmol/min/mg using synthetic Abl peptide (EAIYAAPFAKKK) as substrate.
Protein Construction
A DNA sequence encoding the amino acid sequence (Pro 137-Ser 554) of human ABL1 isoform B (NP_009297.2) was fused with the GST tag at the N-terminus.
Accession#
NP_009297.2
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human ABL1/GST chimera consists of 645 amino acids and predicts a molecular mass of 74 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM EDTA, 0.5mM Reduced Glutathione, pH 8.0Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
c-Abl/ABL1 Protein, Human, Recombinant (GST Tag): Images
c-Abl/ABL1 Protein, Human, Recombinant (GST Tag): Synonyms
ABL Protein, Human; bcr/abl Protein, Human; c-ABL Protein, Human; c-ABL1 Protein, Human; JTK7 Protein, Human; p150 Protein, Human; v-abl Protein, Human
c-Abl/ABL1 Background Information
c-Abl belongs to the class of tyrosine kinases and is the prototype of a subfamily which includes two members, c-Abl and Arg (Abl-related gene). Both proteins are localized at the cell membrane, actin cytoskeleton and cytosol, and c-Abl is present in the nucleus as well. c-Abl is a non-receptor tyrosine kinase that participates in multiple signaling pathways linking the cell surface, cytoskeleton, and the nucleus. Recent in vitro studies have also linked c-Abl to amyloid-beta-induced toxicity and tau phosphorylation. c-Abl has been implicated in many cellular processes including differentiation, division, adhesion, death, and stress response. c-Abl is a latent tyrosine kinase that becomes activated in response to numerous extra- and intra-cellular stimuli. The c-Abl protein is a ubiquitously expressed nonreceptor tyrosine kinase involved in the development and function of many mammalian organ systems, including the immune system and bone. It regulates the cellular response to TAM through functional interaction with the estrogen receptor, which suggests c-Abl as a therapeutic target and a prognostic tumor marker for breast cancer. c-Abl also plays a key role in signaling chemokine-induced T-cell migration. In addition, c-Abl contains NLSs (nuclear localization signals) and DNA-binding sequences important for nuclear functions. c-Abl has become an important therapeutic target in human chronic myeloid leukaemia.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
ABL proto-oncogene 1, non-receptor tyrosine kinase
Research Areas
- Cancer Drug Targets
Related Pathways
Jak-Stat Signaling Pathway
References
- Qiu Z, et al. (2010) c-Abl tyrosine kinase regulates cardiac growth and development. Proc Natl Acad Sci U S A. 107(3): 1136-41.
- Huang Y, et al. (2008) The c-Abl tyrosine kinase regulates actin remodeling at the immune synapse. Blood. 112(1): 111-9.
- Sirvent A, et al. (2008) Cytoplasmic signalling by the c-Abl tyrosine kinase in normal and cancer cells. Biol Cell. 100(11): 617-31.
- Shaul Y, et al. (2005) Role of c-Abl in the DNA damage stress response. Cell Res. 15(1): 33-5.
- A sensitive and microscale method for drug screening combining affinity probes and single molecule fluorescence correlation spectroscopyAuthorRuan, L;Su, D;Shao, C;Wang, J;Dong, C;Huang, X;Ren, J;Year2015JournalAnalystApplicationbindingPubMed ID: 25526365FoldedExpand
- Screening of break point cluster region Abelson tyrosine kinase inhibitors by capillary electrophoresisAuthorXu, M;Zheng, M;Liu, G;Zhang, M;Kang, J;Year2018JournalJ Chromatogr AApplicationenzymePubMed ID: 29336809FoldedExpand
- Simple and Sensitive Method for Determination of Protein Kinase Activity Based on Surface Charge Change of Peptide-Modified Gold Nanoparticles As SubstratesAuthorYi, F;Huang, X;Ren, J;Year2018JournalAnal. Chem.ApplicationenzymePubMed ID: 29468867FoldedExpand
- Discovery of berberine that targetedly induce autophagic degradation of both BCR-ABL and BCR-ABL T315I through recruiting LRSAM1 for overcoming imatinib-resistanceAuthorYin, Z;Huang, G;Gu, C;Liu, Y;Yang, J;Fei, J;Year2020JournalClin. Cancer Res.ApplicationenzymePubMed ID: 32098768FoldedExpand
- In Situ Study of the Drug-target Protein Interaction in Single Living Cells by Combining Fluorescence Correlation Spectroscopy with Affinity ProbesAuthorDeng, L;Huang, X;Ren, J;Year2020JournalAnal. Chem.ApplicationFCSPubMed ID: 32340449FoldedExpand
If you couldn"t find the specific citation, you can:
- Find All Citations
- Submit a CitationGet a Publication Reward
- Leave Us a Message
E.coli protein expression service
Mammalian transient protein expression service
Baculovirus-insect expression service
Stable cell line development service (CHO / HEK293)
品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
联络我们
