Sino Biological/CD86 Protein, Human, Recombinant (His & hFc Tag)/1/10699-H03H
商品编号：
10699-H03H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CD86 Protein, Human, Recombinant (His & hFc Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its ability to bind human CD28 in a functional ELISA.2. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells.The ED50 for this effect is 1-5μg/mL.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-His 239) of human B7-2 (NP_008820.2) pro-protein was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
Accession#
NP_008820.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Leu 20
Molecule Mass
The recombinant human B7-2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 467 amino acids and has a predicted molecular mass of 53.2 kDa. In SDS-PAGE, the apparent molecular mass of rh B7-2/Fc monomer is approximately 80-90 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD86 Protein, Human, Recombinant (His & hFc Tag): Images
CD86 Protein, Human, Recombinant (His & hFc Tag): Synonyms
B7-2 Protein, Human; B7.2 Protein, Human; B70 Protein, Human; CD28LG2 Protein, Human; LAB72 Protein, Human
CD86 Background Information
CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
- Cancer Immunotherapy
- Co-inhibitory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: FCM Antibodies
- Immune Checkpoint Detection: IP Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Proteins
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
Full Name
CD86 antigen
Research Areas
- Cancer Drug Targets
References
- Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
- Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.
- Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.
Human CD86 Proteins
- CD86 Protein, Human, Recombinant (His & hFc Tag), Biotinylated10699-H03H-B
- CD86 Protein, Human, Recombinant (His Tag), Biotinylated10699-H08H-B
- CD86 Protein, Human, Recombinant (ECD, hFc Tag)10699-H02H
- CD86 Protein, Human, Recombinant (His Tag)10699-H08H
Mouse CD86 Proteins
- CD86 Protein, Mouse, Recombinant (His Tag), Biotinylated50068-M08H-B
- CD86 Protein, Mouse, Recombinant (ECD, hFc Tag)50068-M02H
- CD86 Protein, Mouse, Recombinant (His Tag)50068-M08H
- CD86 Protein, Mouse, Recombinant (His & hFc Tag)50068-M03H
Rat CD86 Proteins
- CD86 Protein, Rat, Recombinant (His Tag)80025-R08H
Cynomolgus, Rhesus CD86 Proteins
- CD86 Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)90270-C02H
- CD86 Protein, Cynomolgus, Rhesus, Recombinant (His Tag), HPLC-verifiedHPLC-90270-C08H
- CD86 Protein, Cynomolgus, Rhesus, Recombinant (His Tag)90270-C08H
- A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapyAuthorDu, X;Tang, F;Liu, M;Su, J;Zhang, Y;Wu, W;Devenport, M;Lazarski, CA;Zhang, P;Wang, X;Ye, P;Wang, C;Hwang, E;Zhu, T;Xu, T;Zheng, P;Liu, Y;Year2018JournalCell Res.ApplicationbindingPubMed ID: 29472691FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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