Sino Biological/CEACAM1蛋白，人，重组（ECD，His-Tag）/1/10822-H08H
商品编号：
10822-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CEACAM1 Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its ability to bind biotinylated Human CEACAM6-His (Cat:10823-H08H) in functional ELISA.2. Measured by its ability to bind biotinylated Human CEACAM8-His (Cat:11729-H08H) in functional ELISA.3. Measured by its ability to inhibit IL2 secretion by HuT 78 human cutaneous T cell lymphoma cells. The ED50 for this effect is typically 213 µg/mL.
Protein Construction
A DNA sequence encoding the extracellular domain of human CEACAM1 (NP_001020083.1) (Met 1-Gly 428) was expressed with a C-terminal polyhistidine tag.
Accession#
NP_001020083.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 35
Molecule Mass
The secreted recombinant human CEACAM1 comprises 405 amino acids and predicts a molecular mass of 44.8 kDa. As a result of glycosylation, the apparent molecular mass of rhCEACAM1 is approximately 60.5 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CEACAM1 Protein, Human, Recombinant (ECD, His Tag): Images
Measured by its ability to inhibit IL2 secretion by HuT 78 human cutaneous T cell lymphoma cells. The ED50 for this effect is typically 213 µg/mL.
CEACAM1 Protein, Human, Recombinant (ECD, His Tag): Synonyms
BGP Protein, Human; BGP1 Protein, Human; BGPI Protein, Human; CD66a Protein, Human; CEACAM‑1 Protein, Human
CEACAM1 Background Information
The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
- Cancer Immunotherapy
- Co-inhibitory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: FCM Antibodies
- Immune Checkpoint Detection: ICC Antibodies
- Immune Checkpoint Detection: IHC Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Proteins
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
Full Name
carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Research Areas
- Cancer Drug Targets
- ITIM/ITAM Immunoreceptors and Related Molecules
References
- Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.
- Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.
- Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.
Human CEACAM1 Proteins
- CEACAM1 Protein, Human, Recombinant (ECD, His & hFc Tag)10822-H03H
Mouse CEACAM1 Proteins
- CEACAM1 Protein, Mouse, Recombinant (His Tag)50571-M08H
- CEACAM1 Protein, Mouse, Recombinant (ECD, hFc Tag)50571-M02H
Rat CEACAM1 Proteins
- CEACAM1 Protein, Rat, Recombinant (His Tag)80224-R08H
- Thioloxidoreductase HP0231 of Helicobacter pylori impacts HopQ-dependent CagA translocationAuthorGrzeszczuk, MJ;Bocian-Ostrzycka, KM;Banaś, AM;Roszczenko-Jasinska, P;Malinowska, A;Stralova, H;Haas, R;Meyer, TF;Jagusztyn-Krynicka, EK;Year2018JournalInt. J. Med. Microbiol.ApplicationbindingPubMed ID: 30131271FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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