Sino-Biological/GDNF蛋白，人，重组/1/10561-HNCH
商品编号：
10561-HNCH
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
GDNF Protein, Human, Recombinant: Product Information
Purity
> 97 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-20 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 His Chimera.2. Measured by its ability to bind biotinylated Human GFRa1-His in functional ELISA.
Protein Construction
A DNA sequence encoding the mature form of human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.
Accession#
P39905-2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gly
Molecule Mass
The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
GDNF Protein, Human, Recombinant: Images
GDNF Protein, Human, Recombinant: Synonyms
ATF1 Protein, Human; ATF2 Protein, Human; HFB1-GDNF Protein, Human; HSCR3 Protein, Human
GDNF Background Information
Glial cell line-derived neurotrophic factor(GDNF) is an important member of the GDNF family of ligands(GFL). The GDNF family of ligands is comprised by four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN). It has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. As the founding member, GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. It has been shown that GDNF results in two Parkinson"s disease clinical trial and in a number of animal trials. It has been taken as a potent survival factor for central motoneurons.
Full Name
glial cell derived neurotrophic factor
References
- Oppenheim RW, et al. (1995) Developing motor neurons rescued from programmed and axotomy-induced cell death by GDNF. Nature. 373 (6512): 344-6.
- Tomac A, et al. (1995) Protection and repair of the nigrostriatal dopaminergic system by GDNF in vivo. Nature. 373 (6512): 335-9.
- Schindelhauer D, et al. (1996) The gene coding for glial cell line derived neurotrophic factor (GDNF) maps to chromosome 5p12-p13.1. Genomics. 28 (3): 605-7.
- Carnicella S, et al. (2008) GDNF is a fast-acting potent inhibitor of alcohol consumption and relapse. Proc Natl Acad Sci . 105 (23): 8114-9.
Rat GDNF Proteins
- GDNF Protein, Rat, Recombinant (His Tag)80050-R07B
Canine GDNF Proteins
- GDNF Protein, Canine, Recombinant (hFc Tag)70255-D01H
- Androgen Promotes Differentiation of PLZF+ Spermatogonia pool via Indirect Regulatory PatternAuthorWang, J;Li, J;Gu, Y;Xia, Q;Song, W;Zhang, X;Yang, Y;Wang, W;Li, H;Zou, K;Year2018JournalbioRxivFoldedExpand
- In Vitro Modeling of Human Germ Cell Development Using Pluripotent Stem CellsAuthorZhao, Y;Ye, S;Liang, D;Wang, P;Fu, J;Ma, Q;Kong, R;Shi, L;Gong, X;Chen, W;Ding, W;Yang, W;Zhu, Z;Chen, H;Sun, X;Zhu, J;Li, Z;Wang, Y;Year2018JournalStem Cell ReportsPubMed ID: 29398481FoldedExpand
- A bioenergetic shift is required for spermatogonial differentiationAuthorChen, W;Zhang, Z;Chang, C;Yang, Z;Wang, P;Fu, H;Year2020JournalCell DiscoveryApplicationcell-basedFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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