Sino Biological/IL-35 Protein, Human, Recombinant (hFc Tag)/1/10705-H02H
商品编号：
10705-H02H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IL-35 Protein, Human, Recombinant (hFc Tag): Product Information
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human IL35 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the mature form of human IL12 p35 subunit (P29459) (Arg 23-Ser 219) linked by a polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus.
Accession#
NP_005746.2 & P29459
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Arg 21
Molecule Mass
The recombinant human IL35/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 645 amino acids and has a predicted molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL35/Fc monomer is approximately 90-100 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL-35 Protein, Human, Recombinant (hFc Tag): Images
IL-35 Protein, Human, Recombinant (hFc Tag): Synonyms
CLMF Protein, Human; IL-12A Protein, Human; IL-35 Protein, Human; Interleukin-35 Protein, Human; NFSK Protein, Human; NKSF1 Protein, Human; P35 Protein, Human
IL-35 Background Information
IL12A is a subunit of a cytokine that acts on T and natural killer cells and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.
Full Name
interleukin 12A
Related Pathways
IL12 signaling pathway
References
- Wolf S.F., et al.,(1991), Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J. Immunol. 146:3074-3081.
- Gubler U., et al., (1991), Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor.Proc. Natl. Acad. Sci. U.S.A. 88:4143-4147.
- Batten M., et al.,(2007), The biology and therapeutic potential of interleukin 27.J. Mol. Med. 85:661-672.
Human IL-35 Proteins
- IL-35 Protein, Human, RecombinantCT040-H0323H
- IL-35 Protein, Human, Recombinant (Flag & His Tag)CT065-H2508H
- Reduced IL-35 levels are associated with increased platelet aggregation and activation in patients with acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantationAuthorZhang, X;Zhou, Y;Xu, L;Han, W;Chen, H;Chen, Y;Fu, H;Zhou, S;Zhao, J;Wang, Q;Feng, F;Zhu, X;Liu, K;Huang, X;Year2014JournalAnn. Hematol.Applicationcell-basedPubMed ID: 25512184FoldedExpand
- Anti-inflammatory effects of interleukin-35 in acquired aplastic anemiaAuthorYu, W;Ge, M;Lu, S;Shi, J;Li, X;Zhang, J;Wang, M;Huang, J;Shao, Y;Huang, Z;Zhang, J;Nie, N;Zheng, Y;Year2015JournalCytokineApplicationcell culturePubMed ID: 26282938FoldedExpand
- IL-35 Is Involved in the Pathogenesis of Guillain-Barré Syndrome Through Its Influence on the Function of CD4+ T CellsAuthorLiu, H;Zhang, W;Tian, FF;Kun, A;Zhou, WB;Xiao, B;Li, J;Year2015JournalImmunol. Invest.Applicationcell-basedFoldedExpand
- Interleukin 35 may contribute to the loss of immunological self-tolerance in patients with primary immune thrombocytopeniaAuthorSun, T;Zhang, D;Yang, Y;Zhang, X;Lv, C;Fu, R;Lv, M;Liu, W;Chen, Y;Liu, W;Huang, Y;Xue, F;Liu, X;Zhang, L;Li, H;Yang, R;Year2015JournalBr. J. Haematol.Applicationcell-basedPubMed ID: 25640666 FoldedExpand
- IL-35 recombinant protein reverses inflammatory bowel disease and psoriasis through regulation of inflammatory cytokines and immune cellsAuthorWang, Y;Mao, Y;Zhang, J;Shi, G;Cheng, L;Lin, Y;Li, Y;Zhang, X;Zhang, Y;Chen, X;Deng, J;Su, X;Dai, L;Yang, Y;Zhang, S;Yu, D;Wei, Y;Deng, H;Year2017JournalJ. Cell. Mol. Med.Applicationanimal-basedPubMed ID: 29193791FoldedExpand
- Expression of IGF-1, IL-27 and IL-35 Receptors in Adjuvant Induced Rheumatoid Arthritis ModelAuthorAbdi, E;Najafipour, H;Joukar, S;Dabiri, S;Esmaeli-Mahani, S;Abbasloo, E;Houshmandi, N;Afsharipour, A;Year2018JournalIran J ImmunolApplicationanimal-basedPubMed ID: 29549229FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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