Sino Biological/LDLR/LDL Receptor Protein, Mouse, Recombinant (His Tag)/1/50305-M08H
商品编号：
50305-M08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
LDLR/LDL Receptor Protein, Mouse, Recombinant (His Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant mouse LDLR. The EC50 of biotinylated mouse LDLR is 0.173 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain of mouse LDLR (NP_034830.2) precursor (Met 1-Arg 790) with substitution of Val 23 and Gly 27 by Ala 23 and Cys 27 respectively was expressed with a C-terminal polyhistidine tag.
Accession#
NP_034830.2
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Ala 22
Molecule Mass
The secreted recombinant mouse LDLR comprises 780 amino acids with a predicted molecular mass of 85.7 kDa. As a result of glycosylation, it migarates as approximately 120-130 kDa protein in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
LDLR/LDL Receptor Protein, Mouse, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized Rat PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant mouse LDLR . The EC50 of biotinylated mouse LDLR is 0.173 μg/ml.
LDLR/LDL Receptor Protein, Mouse, Recombinant (His Tag): Synonyms
Hlb301 Protein, Mouse
LDLR/LDL Receptor Background Information
LDL Receptor, also known as LDLR, is a mosaic protein that belongs to the Low-density lipoprotein receptor gene family. The low-density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low-density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.
Full Name
low density lipoprotein receptor
References
- Yamamoto T, et al. (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 39(1): 27-38.
- Mao B, et al. (2001) LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature. 411(6835): 321-5.
- Pinson KI, et al. (2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature. 407(6803): 535-8.
Human LDLR/LDL Receptor Proteins
- LDLR/LDL Receptor Protein, Human, Recombinant (193D/A, ECD, His Tag)10231-H08H1
- LDLR/LDL Receptor Protein, Human, Recombinant (His Tag)10231-H08H
- LDLR/LDL Receptor Protein, Human, Recombinant (mFc Tag)10231-H05H
- LDLR/LDL Receptor Protein, Human, Recombinant (His Tag), Biotinylated10231-H08H-B
Mouse LDLR/LDL Receptor Proteins
- LDLR/LDL Receptor Protein, Mouse, Recombinant (His Tag), Biotinylated50305-M08H-B
- Low-density lipoprotein receptor represents an apolipoprotein E-independent pathway of Aβ uptake and degradation by astrocytesAuthorBasak, JM;Verghese, PB;Yoon, H;Kim, J;Holtzman, DM;Year2012JournalJ. Biol. Chem.PubMed ID: 22383525FoldedExpand
- Modulating Amyloid Beta Clearance by Altering Cellular and Whole Brain Apolipoprotein E MetabolismAuthorBasak, JM;Year2014JournalThesisFoldedExpand
- Optimization and in Vivo Validation of Peptide Vectors Targeting the LDL ReceptorAuthorJacquot, G;Lécorché, P;Malcor, JD;Laurencin, M;Smirnova, M;Varini, K;Malicet, C;Gassiot, F;Abouzid, K;Faucon, A;David, M;Gaudin, N;Masse, M;Ferracci, G;Dive, V;Cisternino, S;Khrestchatisky, M;Year2016JournalMol. Pharm.ApplicationSPRPubMed ID: 27656777FoldedExpand
- Identification and characterization of highly versatile peptide-vectors that bind non-competitively to the low-density lipoprotein receptor for in vivo targeting and delivery of small molecules and protein cargosAuthorDavid, M;Lécorché, P;Masse, M;Faucon, A;Abouzid, K;Gaudin, N;Varini, K;Gassiot, F;Ferracci, G;Jacquot, G;Vlieghe, P;Khrestchatisky, M;Year2018JournalPLoS ONEPubMed ID: 29485998FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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