Sino Biological/PARP Protein, Human, Recombinant (His Tag)/1/11040-H08B
商品编号：
11040-H08B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PARP Protein, Human, Recombinant (His Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human PARP His (Cat: 11040-H08B) at 2 μg/ml (100 μl/well) can bind Human HSP70 His (Cat:10001-H08H), biotinylated, the EC50 of HSP70 His, biotinylated is 0.2-1.0 μg/mL.
Protein Construction
The amino acids corresponding to the full length of human PARP1 (NP_001609.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the C-terminus.
Accession#
NP_001609.2
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human PARP1 consists of 1024 amino acids and predicts a molecular mass of 114.5 kDa. The apparent molecular mass of rhPARP1 is approximately 100-110 kDa in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile PBS, pH 7.4Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Liquid. It is shipped out with blue ice.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PARP Protein, Human, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized Human PARP His (Cat: 11040-H08B) at 2 μg/ml (100 μl/well) can bind Human HSP70 His (Cat:10001-H08H), biotinylated, the EC50 of HSP70 His, biotinylated is 0.2-1.0 μg/mL.
PARP Protein, Human, Recombinant (His Tag): Synonyms
ADPRT Protein, Human; ADPRT1 Protein, Human; ARTD1 Protein, Human; pADPRT-1 Protein, Human; PARP Protein, Human; PARP-1 Protein, Human; PPOL Protein, Human
PARP Background Information
Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2"-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
poly (ADP-ribose) polymerase 1
Research Areas
- Cancer Drug Targets
References
- Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843.
- Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
- Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
- Ahell I. et al., 2008, Nature. 451: 81-85.
Mouse PARP Proteins
- PARP Protein, Mouse, Recombinant (His Tag)50753-M07B
- PARP inhibitor with selectivity toward ADP-ribosyltransferase ARTD3/PARP3AuthorLindgren, AE;Karlberg, T;Thorsell, AG;Hesse, M;Spjut, S;Ekblad, T;Andersson, CD;Pinto, AF;Weigelt, J;Hottiger, MO;Linusson, A;Elofsson, M;Schüler, H;Year2013JournalACS Chem. Biol.PubMed ID: 23742272FoldedExpand
- Nucleic Acid-Induced Potentiation of Matrix Metalloproteinase-9 Enzymatic ActivityAuthorDuellman, T;Chen, X;Wakamiya, R;Yang, J;Year2018JournalBiochem. J.ApplicationsubstratePubMed ID: 29654109FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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