Sino Biological/P-Selectin Protein, Mouse, Recombinant (His Tag)/1/50737-M08H
商品编号：
50737-M08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
P-Selectin Protein, Mouse, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 cells.When 5 x 10E4 cells/well are added to SELP-coated plates (5 μg/mL and 100 μL/well), approximately >60% cells will adhere specifically after 60 minutes at 37℃.
Protein Construction
A DNA sequence encoding the mouse SELP (Q01102) (Met1-Ala709) was expressed with a C-terminal polyhistidine tag.
Accession#
Q01102
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Trp 42
Molecule Mass
The recombinant mouse SELP comprises 679 amino acids and has a predicted molecular mass of 74 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
P-Selectin Protein, Mouse, Recombinant (His Tag): Images
P-Selectin Protein, Mouse, Recombinant (His Tag): Synonyms
CD62P Protein, Mouse; GMP-140 Protein, Mouse; Grmp Protein, Mouse; LECAM3 Protein, Mouse; PADGEM Protein, Mouse
P-Selectin Background Information
P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.
Full Name
selectin P (granule membrane protein 140kDa, antigen CD62)
References
- Johnson-Tidey RR, et al. (1994) Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 144(5):952-61.
- Walcheck B, et al. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. J Clin Invest. 98(5):1081-7.
- Foreman KE, et al. (1994) C5a-induced expression of P-selectin in endothelial cells. J Clin Invest. 94(3):1147-55.
Human P-Selectin Proteins
- P-Selectin Protein, Human, Recombinant (His Tag)13025-H08H
- P-Selectin Protein, Human, Recombinant (hFc Tag)13025-H02H
Mouse P-Selectin Proteins
- P-Selectin Protein, Mouse, Recombinant (hFc Tag)50737-M02H
Rat P-Selectin Proteins
- P-Selectin Protein, Rat, Recombinant (hFc Tag)80451-R02H
- P-Selectin Protein, Rat, Recombinant (His Tag)80451-R08H
Rhesus P-Selectin Proteins
- P-Selectin Protein, Rhesus, Recombinant (hFc Tag)90170-C02H
- P-Selectin Protein, Rhesus, Recombinant (His Tag)90170-C08H
- Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates AtherosclerosisAuthorWinter, C;Silvestre-Roig, C;Ortega-Gomez, A;Lemnitzer, P;Poelman, H;Schumski, A;Winter, J;Drechsler, M;de Jong, R;Immler, R;Sperandio, M;Hristov, M;Zeller, T;Nicolaes, GAF;Weber, C;Viola, JR;Hidalgo, A;Scheiermann, C;Soehnlein, O;Year2018JournalCell Metab.PubMed ID: 29861387FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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