Sino Biological/皮质醇结合球蛋白，人，重组（His标签）/1/10998-H08H
商品编号：
10998-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Cortisol Binding Globulin Protein, Human, Recombinant (His Tag): Product Information
Purity
> 98 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human SERPINA6 (NP_001747.2) precursor (Met 1-Val 405) was fused with a polyhistidine tag at the C-terminus.
Accession#
NP_001747.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Met 23
Molecule Mass
The secreted recombinant human SERPINA6 consists of 394 amino acids and predictes a molecular mass of 44 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 55-60 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Cortisol Binding Globulin Protein, Human, Recombinant (His Tag): Images
Cortisol Binding Globulin Protein, Human, Recombinant (His Tag): Synonyms
CBG Protein, Human; SerpinA6 Protein, Human
Cortisol Binding Globulin Background Information
Corticosteroid-binding globulin (CBG), also known as SerpinA6, is a non-inhibitory member of the serine proteinase inhibitor (serpin) superfamily. It is the high-affinity transport protein for glucocorticoids in vertebrate blood. CBG is specifically cleaved by this protease at a precise site close to its carboxy-terminus. This induces a conformation change and disrupts the binding between glucocorticoids and CBG, and promotes a significant and local release of glucocorticoids (over 90% of them are bound to CBG in human plasma). In this context, CBG directs glucocorticoids to sites of inflammation, and plays in consequence a crucial role in efficient glucocorticoid action in physiology. The SerpinA6 protein is mainly secreted by the liver. This negative acute phase protein regulates free cortisol levels in the blood and distributes cortisol to its target tissues. SerpinA6 deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo-/hypertension and muscle fatigue. There are three heritable, human CBG gene mutations that can reduce CBG-cortisol binding affinity and/or reduce circulating CBG levels.
Full Name
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
References
- Seralini GE. (1991) A new role for corticosteroid binding globulin (CBG), member of SERPIN superfamily. C R Seances Soc Biol Fil. 185(6): 500-9.
- Buss C, et al. (2007) Haploinsufficiency of the SERPINA6 gene is associated with severe muscle fatigue: A de novo mutation in corticosteroid-binding globulin deficiency. J Neural Transm. 114(5): 563-9.
- Torpy DJ, et al. (2007) Corticosteroid-binding globulin gene polymorphisms: clinical implications and links to idiopathic chronic fatigue disorders. Clin Endocrinol (Oxf). 67(2): 161-7.
- Braun BC, et al. (2010) Effect of mutations of the human serpin protein corticosteroid-binding globulin on cortisol-binding, thermal and protease sensitivity. J Steroid Biochem Mol Biol. 120(1): 30-7.
- Lin HY, et al. (2010) Molecular and structural basis of steroid hormone binding and release from corticosteroid-binding globulin. Mol Cell Endocrinol. 316(1): 3-12.
Human Cortisol Binding Globulin Proteins
- Cortisol Binding Globulin Protein, Human, Recombinant (His & AVI Tag), Biotinylated10998-H27H-B
Mouse Cortisol Binding Globulin Proteins
- Cortisol Binding Globulin Protein, Mouse, Recombinant (His Tag)50314-M08H
- Corticosteroid-binding globulin cleavage is paradoxically reduced in alpha-1 antitrypsin deficiency: Implications for cortisol homeostasisAuthorNenke, MA;Holmes, M;Rankin, W;Lewis, JG;Year2016JournalClinica Chimica ActaApplicationstandardFoldedExpand
- Corticosteroid-binding globulin cleavage may be pathogen-dependent in bloodstream infectionAuthorNenke, MA;Lewis, JG;Rankin, W;Shaw, D;Torpy, DJ;Year2016JournalClin. Chim. ActaApplicationstandardPubMed ID: 27887960FoldedExpand
- Evidence of Reduced CBG Cleavage in Abdominal Obesity: A Potential Factor in Development of the Metabolic SyndromeAuthorNenke, MA;Lewis, JG;Rankin, W;Torpy, DJ;Year2016JournalHorm. Metab. Res.ApplicationbindingPubMed ID: 27756081FoldedExpand
- Reduced corticosteroid-binding globulin cleavage in active rheumatoid arthritisAuthorNenke, MA;Lewis, JG;Rankin, W;McWilliams, L;Metcalf, RG;Proudman, SM;Torpy, DJ;Year2016JournalClin. Endocrinol. (Oxf)ApplicationELISA (standard)PubMed ID: 27061835FoldedExpand
- Total and high-affinity corticosteroid-binding globulin depletion in septic shock is associated with mortalityAuthorMeyer, EJ;Nenke, MA;Rankin, W;Lewis, JG;Konings, E;Slager, M;Jansen, TC;Bakker, J;Hofland, J;Feelders, RA;Torpy, DJ;Year2018JournalClin. Endocrinol. (Oxf)ApplicationELISA (standard)PubMed ID: 30160799FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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