Sino Biological/UCHL1 Protein, Mouse, Recombinant (His Tag)/1/50690-M07E
商品编号：
50690-M07E
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
UCHL1 Protein, Mouse, Recombinant (His Tag): Product Information
Purity
> 97 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.
Protein Construction
A DNA sequence encoding the mouse UCHL1 (Q9R0P9) (Gln 2-Ala 223) was expressed, with a polyhistide tag at the N-terminus.
Accession#
Q9R0P9
Expressed Host
E. coli
Species
Mouse
Predicted N Terminal
Met
Molecule Mass
The recombinant mouse UCHL1 consisting of 233 amino acids and has a calculated molecular mass of 26.2 kDa. rm UCHL1 migrates as an approximately 29 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 50mM Tris, 150mM NaCl, 20% glycerol, pH 7.7Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
UCHL1 Protein, Mouse, Recombinant (His Tag): Images
UCHL1 Protein, Mouse, Recombinant (His Tag): Synonyms
AW822034 Protein, Mouse; C88048 Protein, Mouse; gad Protein, Mouse; PGP9.5 Protein, Mouse; R75593 Protein, Mouse; UCH-L1 Protein, Mouse; UCHL-1 Protein, Mouse
UCHL1 Background Information
Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson"s, Alzheimer"s, and Huntington"s disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.
Full Name
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
References
- Wada H., et al., 1998, Biochem. Biophys. Res. Commun. 251:688-92.
- Choi J., et al., 2004, J. Biol. Chem. 279:13256-64.
- Lombardino,A.2005, et al., J.Proc Natl Acad Sci.USA102(22):8036-41
- Okochi-Takada,E. et al., 2006, Int J Cancer. 119(6):1338-44.
- Zetterberg, M. et al., 2010, Mol Neurodegener5:11.
Human UCHL1 Proteins
- UCHL1 Protein, Human, Recombinant (His Tag)11663-H07E
Rat UCHL1 Proteins
- UCHL1 Protein, Rat, Recombinant (His Tag)80089-R07E
- Identification of Novel Protein Targets of Dimethyl Fumarate Modification in Neurons and Astrocytes Reveals Actions Independent of Nrf2 StabilizationAuthorPiroli, GG;Manuel, AM;Patel, T;Walla, MD;Shi, L;Lanci, SA;Wang, J;Galloway, A;Ortinski, PI;Smith, DS;Frizzell, N;Year2018JournalMol. Cell ProteomicsApplicationenzymePubMed ID: 30587509FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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