Sino Biological/GM-CSF Protein, Mouse, Recombinant (His Tag)/1/51048-M07H
商品编号：
51048-M07H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
GM-CSF Protein, Mouse, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured in a cell proliferation assay using FDC-P1 cells.The ED50 for this effect is typically 0.04-0.2 ng/mL.
Protein Construction
A DNA sequence encoding the mouse CSF2 (P01587) (Ala18-Lys141) was expressed with a polyhistidine tag at the N-terminus.
Accession#
P01587
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
His
Molecule Mass
The recombinant mouse CSF2 consists of 144 amino acids and predicts a molecular mass of 16.5 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
GM-CSF Protein, Mouse, Recombinant (His Tag): Images
Measured in a cell proliferation assay using FDC-P1 cells.The ED50 for this effect is typically 0.04-0.2 ng/mL.
GM-CSF Protein, Mouse, Recombinant (His Tag): Synonyms
Csfgm Protein, Mouse; Gm-CSf Protein, Mouse; GMCSF Protein, Mouse; MGI-IGM Protein, Mouse
GM-CSF Background Information
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent development. Several cell lineages in the reproductive tract and gestational tissues synthesise GM-CSF under direction by ovarian steroid hormones and signalling agents originating in male seminal fluid and the conceptus. The pre-implantation embryo, invading placental trophoblast cells and the abundant populations of leukocytes controlling maternal immune tolerance are all subject to GM-CSF regulation. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 (cellular) immune responses in cognate T cells. The active form of the protein is found extracellularly as a homodimer, and the encoding gene is localized to a related gene cluster at chromosome region 5q31 which is known to be associated with 5q-syndrome and acute myelogenous leukemia. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. While the benefits of GM-CSF in this arena have been promising, recent reports have suggested the potential for GM-CSF to induce immune suppression and, thus, negatively impact outcomes in the management of cancer patients. GM-CSF deficiency in pregnancy adversely impacts fetal and placental development, as well as progeny viability and growth after birth, highlighting this cytokine as a central maternal determinant of pregnancy outcome with clinical relevance in human fertility.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
colony stimulating factor 2 (granulocyte-macrophage)
Research Areas
- Cancer Drug Targets
Related Pathways
IL17 signaling pathway
References
- Robertson SA. (2007) GM-CSF regulation of embryo development and pregnancy. Cytokine Growth Factor Rev. 18(3-4): 287-98.
- Waller EK. (2007) The role of sargramostim (rhGM-CSF) as immunotherapy. Oncologist. 12 Suppl 2: 22-6.
- Clive KS, et al. (2010) Use of GM-CSF as an adjuvant with cancer vaccines: beneficial or detrimental? Expert Rev Vaccines. 9(5): 519-25.
Human GM-CSF Proteins
- GM-CSF Protein, Human, RecombinantGMP-10015-HNAH
- GM-CSF Protein, Human, Recombinant (His Tag)10015-H07H
- GM-CSF Protein, Human, Recombinant, HPLC-verifiedHPLC-10015-HNAH
- GM-CSF Protein, Human, Recombinant, Biotinylated10015-HNAH-B
- GM-CSF Protein, Human, Recombinant (hFc Tag)10015-H01H
- GM-CSF Protein, Human, Recombinant10015-HNAH
- GM-CSF Protein, Human, Recombinant10015-HNAY
Mouse GM-CSF Proteins
- GM-CSF Protein, Mouse, Recombinant51048-MNAH
- GM-CSF Protein, Mouse, Recombinant (hFc Tag)51048-M01H
Rat GM-CSF Proteins
- GM-CSF Protein, Rat, Recombinant (His Tag)80020-R07H
- GM-CSF Protein, Rat, Recombinant (hFc Tag)80020-R01H
- Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cellsAuthorWang, H;Lin, J;Zeng, L;Ouyang, C;Ran, P;Yang, P;Liu, Z;Year2017JournalSci RepApplicationbone marrow cells culture,BMDCsPubMed ID: 28819104FoldedExpand
- Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse modelAuthorWang, H;Mo, L;Xiao, X;An, S;Liu, X;Ba, J;Wu, W;Ran, P;Yang, P;Liu, Z;Year2017JournalSci RepApplicationcell-based (culture)PubMed ID: 28240301FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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