Sino Biological/Ephrin A5蛋白，人，重组（His-Tag）/1/10192-H08H
商品编号：
10192-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Ephrin A5 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized human EPHA5 at 20 μg/ml (100 μl/well) can bind human EFNA4 with a linear range of 1.28-32 ng/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Asn 203) of human Ephrin-A5 (NP_001953.1) precursor was expressed with a C-terminal polyhistidine tag.
Accession#
NP_001953.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 21
Molecule Mass
The recombinant human Ephrin-A5 consists of 194 amino acids and has a calculated molecular mass of 23 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphrin-A5 is approximately 27 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Ephrin A5 Protein, Human, Recombinant (His Tag): Images
Ephrin A5 Protein, Human, Recombinant (His Tag): Synonyms
AF1 Protein, Human; EFL5 Protein, Human; EPLG7 Protein, Human; GLC1M Protein, Human; LERK7 Protein, Human; RAGS Protein, Human
Ephrin A5 Background Information
Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
Full Name
ephrin-A5
References
- Frisén J, et al. (1998) Ephrin-A5 (AL-1/RAGS) is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system. Neuron. 20(2): 235-43.
- Feldheim DA, et al. (2000) Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping. Neuron. 25(3): 563-74.
- Wahl S, et al. (2000) Ephrin-A5 induces collapse of growth cones by activating Rho and Rho kinase. J Cell Biol. 149(2): 263-70.
Human Ephrin A5 Proteins
- Ephrin A5 Protein, Human, Recombinant (hFc Tag)10192-H02H
Mouse Ephrin A5 Proteins
- Ephrin A5 Protein, Mouse, Recombinant (His Tag)50597-M08H
- Ephrin A5 Protein, Mouse, Recombinant (hFc Tag)50597-M02H
Rat Ephrin A5 Proteins
- Ephrin A5 Protein, Rat, Recombinant (hFc Tag)80105-R02H
- Ephrin A5 Protein, Rat, Recombinant (His Tag)80105-R08H
Rhesus Ephrin A5 Proteins
- Ephrin A5 Protein, Rhesus, Recombinant (hFc Tag)90044-C02H
- Ephrin A5 Protein, Rhesus, Recombinant (His Tag)90044-C08H
Canine Ephrin A5 Proteins
- Ephrin A5 Protein, Canine, Recombinant (hFc Tag)70072-D02H
- Identification and Characterisation of Nanobodies targeting the EphA4 receptorAuthorSchoonaert, L;Rué, L;Roucourt, B;Timmers, M;Little, S;Chávez Gutiérrez, L;Dewilde, M;Joyce, P;Curnock, A;Weber, P;Haustraete, J;Hassanzadeh-Ghassabeh, G;De Strooper, B;Van Den Bosch, L;Van Damme, P;Lemmens, R;Robberecht, W;Year2017JournalJ. Biol. Chem.ApplicationAlphascreen assay to the test the inhibition of EphA7 and ephrin-A5 interaction ephrin-A5 interaction of EphA7 and ephrin-A5 interactionPubMed ID: 28526745FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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