Sino Biological/DR5/TRAIL R2蛋白，小鼠，重组（His-Tag）/1/50412-M08H
商品编号：
50412-M08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
DR5/TRAIL R2 Protein, Mouse, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Immobilized mouse TNFRSF10B-His at 10 μg/ml (100 μl/well) can bind biotinylated human TNFSF10 (Cat:10409-HNAE), The EC50 of biotinylated human TNFSF10 (Cat:10409-HNAE) is 0.16-0.38 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain of mouse TNFRSF10B (NP_064671.2) (Met 1-Ser 177) was expressed, with a C-terminal polyhistidine tag.
Accession#
NP_064671.2
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Asn 53
Molecule Mass
The secreted recombinant mouse TNFRSF10B consists of 136 amino acids and has a calculated molecular mass of 15 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 25-35 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
DR5/TRAIL R2 Protein, Mouse, Recombinant (His Tag): Images
DR5/TRAIL R2 Protein, Mouse, Recombinant (His Tag): Synonyms
DR5 Protein, Mouse; KILLER Protein, Mouse; Ly98 Protein, Mouse; MK Protein, Mouse; TRAILR2 Protein, Mouse; TRICK2A Protein, Mouse; TRICK2B Protein, Mouse; TRICKB Protein, Mouse
DR5/TRAIL R2 Background Information
Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
tumor necrosis factor receptor superfamily, member 10b
Research Areas
- Cancer Drug Targets
Related Pathways
Death Receptor Signaling
p53 Pathway
References
- Schneider P, et al. (1997) TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-kappaB. Immunity. 7(6): 831-6.
- Ichikawa K, et al. (2003) TRAIL-R2 (DR5) mediates apoptosis of synovial fibroblasts in rheumatoid arthritis. J Immunol. 171(2): 1061-9.
- Walczak H, et al. (1997) TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL. EMBO J. 16(17): 5386-97.
Human DR5/TRAIL R2 Proteins
- DR5/TRAIL R2 Protein, Human, Recombinant (His Tag)10465-H08H
- DR5/TRAIL R2 Protein, Human, Recombinant (ECD, hFc Tag)10465-H02H
- DR5/TRAIL R2 Protein, Human, Recombinant (His & hFc Tag)10465-H03H
- DR5/TRAIL R2 Protein, Human, Recombinant (His Tag), Biotinylated10465-H08H-B
Mouse DR5/TRAIL R2 Proteins
- DR5/TRAIL R2 Protein, Mouse, Recombinant (His & hFc Tag)50412-M03H
- Multivalent antibody-scTRAIL fusion proteins for tumor therapy: impact of format and targetingAuthorHutt, M;Year2017JournalThesisApplicationELISAFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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