Sino Biological/Pleiotrophin/PTN Protein, Human, Recombinant/1/10288-HNAB
商品编号：
10288-HNAB
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Pleiotrophin/PTN Protein, Human, Recombinant: Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized human PTN (Cat:10288-HNAB) at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc (Cat:80344-R02H), The EC50 of rat SDC1-Fc (Cat:80344-R02H) is 0.35-0.81 μg/ml.
Protein Construction
A DNA sequence encoding the mature form of human PTN (NP_002816.1) (Met1-Asp168) was expressed.
Accession#
NP_002816.1
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human PTN consists of 136 amino acids and predicts a molecular mass of 15.3 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 1M NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Pleiotrophin/PTN Protein, Human, Recombinant: Images
Measured by its binding ability in a functional ELISA. Immobilized human PTN (Cat: 10288-HNAB) at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc (Cat: 80344-R02H), The EC50 of rat SDC1-Fc (Cat: 80344-R02H) is 0.35-0.81 μg/ml.
Pleiotrophin/PTN Protein, Human, Recombinant: Synonyms
HARP Protein, Human; HBGF8 Protein, Human; HBNF Protein, Human; NEGF1 Protein, Human
Pleiotrophin/PTN Background Information
HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damage in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.
Full Name
pleiotrophin
References
- Vanderwinden JM, et al. (1992) Cellular distribution of the new growth factor pleiotrophin (HB-GAM) mRNA in developing and adult rat tissues. Anat Embryol. 186(4):387-406.
- Lauri SE, et al. (1996) Activity-induced enhancement of HB-GAM expression in rat hippocampal slices. Neuroreport. 7(10):1670-4.
- Pavlov I, et al. (2002) Role of heparin-binding growth-associated molecule (HB-GAM) in hippocampal LTP and spatial learning revealed by studies on overexpressing and knockout mice. Mol Cell Neurosci. 20(2):330-42.
Mouse Pleiotrophin/PTN Proteins
- Pleiotrophin/PTN Protein, Mouse, Recombinant51000-MNAB
- Pleiotrophin/PTN Protein, Mouse, Recombinant (hFc Tag)51000-M01H
- Pathogenic role and therapeutic potential of pleiotrophin in mouse models of ocular vascular diseaseAuthorWang, W;LeBlanc, ME;Chen, X;Chen, P;Ji, Y;Brewer, M;Tian, H;Spring, SR;Webster, KA;Li, W;Year2017JournalAngiogenesisApplicationcell-basedPubMed ID: 28447229FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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