Sino Biological/ERK2 Protein, Human, Recombinant (GST Tag)/1/10030-H09B
商品编号：
10030-H09B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
ERK2 Protein, Human, Recombinant (GST Tag): Product Information
Purity
> 98 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
No Kinase Activity
Protein Construction
A DNA sequence encoding the human ERK2 (NP_002736.3) (Met 1-Ser 360) was fused with the GST tag at the N-terminus.
Accession#
NP_002736.3
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human ERK2/GST chimera consists of 585 amino acids and predicts a molecular mass of 67 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10% Glycerol, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
ERK2 Protein, Human, Recombinant (GST Tag): Images
ERK2 Protein, Human, Recombinant (GST Tag): Synonyms
ERK Protein, Human; ERK-2 Protein, Human; ERK2 Protein, Human; ERT1 Protein, Human; MAPK2 Protein, Human; p38 Protein, Human; p40 Protein, Human; p41 Protein, Human; p41mapk Protein, Human; p42-MAPK Protein, Human; P42MAPK Protein, Human; PRKM1 Protein, Human; PRKM2 Protein, Human
ERK2 Background Information
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. ERK is a versatile protein kinase that regulates many cellular functions. Growing evidence suggests that extracellular signal-regulated protein kinase 1/2 (ERK1/2) plays a crucial role in promoting cell death in a variety of neuronal systems, including neurodegenerative diseases. It is believed that the magnitude and the duration of ERK1/2 activity determine its cellular function. Activation of ERK1/2 is implicated in the pathophysiology of spinal cord injury (SCI). ERK2 signaling is a novel target associated with the deleterious consequences of spinal injury. ERK-2, also known as mitogen-activated protein kinase 1 (MAPK1), is a member of the protein kinase superfamily and MAP kinase subfamily. MKP-3 is a dual-specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases. Upon activation, MAPK1 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. MAPK1 is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating some transcription factors such as ELK1. MAPK1 acts as a transcriptional repressor that represses the expression of interferon gamma-induced genes. Transcriptional activity is independent of kinase activity. The nuclear-cytoplasmic distribution of ERK2 is regulated in response to various stimuli and changes in a cell context. Furthermore, the nuclear flux of ERK2 occurs by several energy- and carrier-dependent and -independent mechanisms. ERK2 has been shown to translocate into and out of the nucleus by facilitated diffusion through the nuclear pore, interacting directly with proteins within the nuclear pore complex, as well as by karyopherin-mediated transport. ERK2 interacts with the PDE4 catalytic unit by binding to a KIM (kinase interaction motif) docking site located on an exposed beta-hairpin loop and an FQF (Phe-Gln-Phe) specificity site located on an exposed alpha-helix. These flank a site that allows phosphorylation by ERK, the functional outcome of which is orchestrated by the N-terminal UCR1/2 (upstream conserved region 1 and 2) modules.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
mitogen-activated protein kinase 1
Research Areas
- Cancer Drug Targets
Related Pathways
Death Receptor Signaling
EGFR Signaling Pathway
NF-kB (NFkB) Pathway
Non-Canonical Wnt Pathway
p53 Pathway
TGF-beta Signaling
TNF Signaling
VEGF Signaling Pathway
AMPK Signaling Pathway
B Cell Receptor Signaling Pathway
G Protein-coupled Receptors Signaling
Jak-Stat Signaling Pathway
MAPK-Erk Pathway
T Cell Receptor Signaling Pathway
Common G-Chain signaling pathway
IL1 signaling pathway
IL17 signaling pathway
References
- Houslay MD, et al. (2003) The role of ERK2 docking and phosphorylation of PDE4 cAMP phosphodiesterase isoforms in mediating cross-talk between the cAMP and ERK signalling pathways. Biochem Soc Trans. 31(Pt 6): 1186-90.
- Jivan A, et al. (2010) Reconstitution of the Nuclear Transport of the MAP Kinase ERK2. Methods Mol Biol. 661: 273-85.
- Yu CG, et al. (2010) Involvement of ERK2 in traumatic spinal cord injury. J Neurochem. 113(1): 131-42.
- Subramaniam S, et al. (2010) ERK and cell death: ERK1/2 in neuronal death. FEBS J. 277(1): 22-9.
Mouse ERK2 Proteins
- ERK2 Protein, Mouse, Recombinant (His & GST Tag)50445-M20B
- ERK2 Protein, Mouse, Recombinant50445-MNCB
- PPARγ Recruitment to Active ERK during Memory Consolidation Is Required for Alzheimer"s Disease-Related Cognitive EnhancementAuthorJB Jahrling, CM Hernandez, L Denner…Year2014JournalThe Journal of neuroscienceApplicationGST-pull downPubMed ID: 24623782FoldedExpand
- PPAR纬-pERK interaction restores memory consolidation in an Alzheimer"s disease modelAuthorJahrling, J;Year2017JournalThesisApplicationGST pull-down assayFoldedExpand
- Revealing active ingredients, potential targets, and action mechanism of Ermiao fang for treating endometritis based on network pharmacology strategyAuthorZhang, C;Su, T;Yu, D;Wang, F;Yue, C;Wang, HQ;Year2020JournalJ EthnopharmacolPubMed ID: 32505843FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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