Sino Biological/IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag)/1/51084-M08B
商品编号：
51084-M08B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His (Cat:51084-M08B) at 10 μg/mL (100 μl/well) can bind rat IL4R-Fc (Cat:80198-R02H), The EC50 of rat IL4R-Fc (Cat:80198-R02H) is 0.12-0.29 μg/mL.2. Measured by its ability to inhibit mIL4-dependent proliferation of HT-2 cells. The ED50 for this effect is typically 2-10 ng/ml in the presence of 2 ng/ml Recombinant Mouse IL4.
Protein Construction
A DNA sequence encoding the mouse IL4 (P07750) (Met 1-Ser140, Q136D, Y139D) was expressed with a polyhistidine tag at the C-terminus.
Accession#
P07750
Expressed Host
Baculovirus-Insect Cells
Species
Mouse
Predicted N Terminal
His 21
Molecule Mass
The recombinant mouse IL4 consists of 131 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag): Images
IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His Tag): Synonyms
BSF-1 Protein, Mouse; Il-4 Protein, Mouse
IL-4 Background Information
Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
interleukin 4
Related Pathways
Common G-Chain signaling pathway
References
- Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
- Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
- Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.
Human IL-4 Proteins
- IL-4 Protein, Human, Recombinant, BiotinylatedGMP-11846-HNAE-B
- IL-4 Protein, Human, RecombinantGMP-11846-HNAE
- IL-4 Protein, Human, Recombinant11846-HNAE
Mouse IL-4 Proteins
- IL-4 Protein, Mouse, Recombinant51084-MNAE
Canine IL-4 Proteins
- IL-4 Protein, Canine, Recombinant70021-DNAE
- Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cellsAuthorWang, H;Lin, J;Zeng, L;Ouyang, C;Ran, P;Yang, P;Liu, Z;Year2017JournalSci RepPubMed ID: 28819104FoldedExpand
- Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse modelAuthorWang, H;Mo, L;Xiao, X;An, S;Liu, X;Ba, J;Wu, W;Ran, P;Yang, P;Liu, Z;Year2017JournalSci RepApplicationcell-based (culture)PubMed ID: 28240301FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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