Sino Biological/TIE2 Protein, Human, Recombinant (ECD, His & hFc Tag)/1/10700-H03H
商品编号：
10700-H03H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TIE2 Protein, Human, Recombinant (ECD, His & hFc Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10 μg/ml (100 μl/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met1-Lys745) of human Tie2 (AAA61139.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Accession#
NP_000450.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 23
Molecule Mass
The recombinant human Tie2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 970 amino acids and predicts a molecular mass of 108.5 kDa. As a result of glycosylation, the apparent molecular mass of rhTie2/Fc monomer is approximately 130.7 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TIE2 Protein, Human, Recombinant (ECD, His & hFc Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10 μg/ml (100 μl/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 μg/ml.
TIE2 Protein, Human, Recombinant (ECD, His & hFc Tag): Synonyms
CD202B Protein, Human; TIE-2 Protein, Human; TIE2 Protein, Human; VMCM Protein, Human; VMCM1 Protein, Human
TIE2 Background Information
TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Full Name
TEK tyrosine kinase, endothelial
Research Areas
- Receptor Tyrosine Kinases (RTKs)
- Cancer Drug Targets
- Hemangioblast Markers
References
- Jones N, et al. (1998) The Tek / Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene. 17(9): 1097-108.
- Sato A, et al. (1998) Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in human hematopoietic progenitor cells. Int Immunol. 10(8): 1217-27.
- Huang L, et al. (1995) GRB2 and SH-PTP2: potentially important endothelial signaling molecules downstream of the TEK / TIE2 receptor tyrosine kinase. Oncogene. 11(10): 2097-103.
Human TIE2 Proteins
- TIE2 Protein, Human, Recombinant (His & GST Tag)10700-H20B1
- TIE2 Protein, Human, Recombinant (ECD, His Tag)10700-H08H
- TIE2 Protein, Human, Recombinant (ECD, His Tag), Biotinylated10700-H08H-B
Mouse TIE2 Proteins
- TIE2 Protein, Mouse, Recombinant (aa 770-1122, His & GST Tag)51087-M20B1
- TIE2 Protein, Mouse, Recombinant (His Tag)51087-M08H
- TIE2 Protein, Mouse, Recombinant (ECD, hFc Tag)51087-M02H
Rat TIE2 Proteins
- TIE2 Protein, Rat, Recombinant (hFc Tag)80063-R02H
Cynomolgus TIE2 Proteins
- TIE2 Protein, Cynomolgus, Recombinant (hFc Tag)90292-C02H
Rhesus TIE2 Proteins
- TIE2 Protein, Rhesus, Recombinant (His Tag)90292-C08H
- Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapyAuthorShlamkovich, T;Aharon, L;Barton, WA;Papo, N;Year2017JournalOncotargetApplicationSPRPubMed ID: 28422724FoldedExpand
- Identification of specific Tie2 cleavage sites and therapeutic modulation in experimental sepsisAuthorIdowu, TO;Etzrodt, V;Seeliger, B;Bolanos-Palmieri, P;Thamm, K;Haller, H;David, S;Year2020JournalElifeApplicationsubstratePubMed ID: 32838837FoldedExpand
If you couldn"t find the specific citation, you can:
- Find All Citations
- Submit a CitationGet a Publication Reward
- Leave Us a Message
E.coli protein expression service
Mammalian transient protein expression service
Baculovirus-insect expression service
Stable cell line development service (CHO / HEK293)
品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
联络我们
