Sino Biological/CD64蛋白，小鼠，重组（His&；AVI标签），生物素化/1/50086-M27H-B
商品编号：
50086-M27H-B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CD64 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Biotin/Protein Ratio
0.7-1 as determined by the HABA assay.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human IgG1 at 10 μg/ml (100 μl/well) can bind biotinylated mouse CD64-AVI. The EC50 of biotinylated human IgG1 is 0.1-0.5 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Accession#
NP_034316.1
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Glu 25
Molecule Mass
The recombinant mouse FCGR1 consists of 308 amino acids after removal of the signal peptide and has a predicted molecular mass of 34.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 50-55 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD64 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Images
Measured by its binding ability in a functional ELISA. Immobilized human IgG1 at 10 μg/ml (100 μl/well) can bind biotinylated mouse CD64-AVI , The EC50 of biotinylated human IgG1 is 0.1-0.5 μg/ml.
CD64 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Synonyms
AI323638 Protein, Mouse; AV092959 Protein, Mouse; CD64 Protein, Mouse; FcgammaRI Protein, Mouse; IGGHAFC Protein, Mouse
CD64 Background Information
High affinity immunoglobulingamma Fc receptor I, also known as FCGR1 and CD64, is an integralmembraneglycoprotein and a member of the immunoglobulin superfamily. CD64is a high affinity receptor for the Fc region of IgG gamma and functions inboth innate and adaptive immune responses. Receptors that recognize the Fcportion of IgG function in the regulation of immune response and are dividedinto three classes designated CD64, CD32, and CD16. CD64 is structurallycomposed of asignal peptidethat allows its transport to the surfaceof a cell, threeextracellularimmunoglobulin domainsof theC2-type that it uses to bind antibody, a hydrophobictransmembrane domain,and a short cytoplasmic tail. CD64 isconstitutivelyfound on onlymacrophages and monocytes, but treatment of polymorphonuclearleukocyteswith cytokines likeIFNγandG-CSFcaninduce CD64 expression on these cells. The inactivation of the mouse CD64resulted in a wide range of defects in antibody Fc-dependent functions. MouseCD64 is an early participant in Fc-dependent cell activation and in thedevelopment of immune responses.
Full Name
Fc receptor, IgG, high affinity I
Research Areas
- Fc Receptors
References
- Allen J.M., et al.,(1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824.
- Allen J.M., et al., (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381.
- Porges A.J., et al.,(1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.
Human CD64 Proteins
- CD64 Protein, Human, Recombinant (His Tag), Biotinylated10256-H08S-B
- CD64 Protein, Human, Recombinant (ECD, His Tag)10256-H08S
- CD64 Protein, Human, Recombinant (His & AVI Tag), Biotinylated10256-H27H-B
- CD64 Protein, Human, Recombinant (ECD, His Tag)10256-H08H
Mouse CD64 Proteins
- CD64 Protein, Mouse, Recombinant (His Tag)50086-M08H
- CD64 Protein, Mouse, Recombinant (His & AVI Tag)50086-M27H
Rat CD64 Proteins
- CD64 Protein, Rat, Recombinant (His Tag)80016-R08H
- Conserved FcγR- glycan discriminates between fucosylated and afucosylated IgG in humans and mice.AuthorDekkers, G;Bentlage, AEH;Plomp, R;Visser, R;Koeleman, CAM;Beentjes, A;Mok, JY;van Esch, WJE;Wuhrer, M;Rispens, T;Vidarsson, G;Year2017JournalMol. Immunol.ApplicationSPRPubMed ID: 29268168FoldedExpand
- Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activationAuthorZeeuw van der Laan, EAN;van der Velden, S;Bentlage, AEH;Larsen, MD;van Osch, TLJ;Mok, JY;Brasser, G;Geerdes, DM;Koeleman, CAM;Nouta, J;Semple, JW;Porcelijn, L;van Esch, WJE;Wuhrer, M;van der Schoot, CE;Vidarsson, G;Kapur, R;Year2020JournalBlood AdvApplicationSPRPubMed ID: 32810222FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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