Sino Biological/FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated/1/50036-M27H-B
商品编号：
50036-M27H-B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Biotin/Protein Ratio
0.5-1 as determined by the HABA assay.
Activity
1. Measured by its ability to bind human IgG1 (Fc) (Cat:10702-HNAC) in a functional ELISA.2. Using the Octet RED System, the affinity constant (Kd) of FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated (Cat. 50036-M27H-B) bound to IgG1 Antibody was 0.03 μM.
Protein Construction
The amino acid sequence corronding to (Met 1-Gln 203) of mouse FCGR4 (NP_653142.2) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Accession#
NP_653142.2
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Glu 19
Molecule Mass
The recombinant mouse FCGR4 consists of 214 amino acids and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 35-40 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Images
Measured by its ability to bind human IgG1 (Fc) (Cat: 10702-HNAC) in a functional ELISA.
Using the Octet RED System, the affinity constant (Kd) of FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated (Cat. 50036-M27H-B) bound to IgG1 Antibody was 0.03 μM.
FCGR4 Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated: Synonyms
4833442P21Rik Protein, Mouse; CD16-2 Protein, Mouse; FcgammaRIV Protein, Mouse; Fcgr3a Protein, Mouse; FcgRIV Protein, Mouse; Fcrl3 Protein, Mouse
FCGR4 Background Information
Full Name
Fc receptor, IgG, low affinity IV
Research Areas
- Fc Receptors
Mouse FCGR4 Proteins
- FCGR4 Protein, Mouse, Recombinant (His Tag)50036-M08H
- FCGR4 Protein, Mouse, Recombinant (His & AVI Tag)50036-M27H
- Conserved FcγR- glycan discriminates between fucosylated and afucosylated IgG in humans and mice.AuthorDekkers, G;Bentlage, AEH;Plomp, R;Visser, R;Koeleman, CAM;Beentjes, A;Mok, JY;van Esch, WJE;Wuhrer, M;Rispens, T;Vidarsson, G;Year2017JournalMol. Immunol.PubMed ID: 29268168FoldedExpand
- Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activationAuthorZeeuw van der Laan, EAN;van der Velden, S;Bentlage, AEH;Larsen, MD;van Osch, TLJ;Mok, JY;Brasser, G;Geerdes, DM;Koeleman, CAM;Nouta, J;Semple, JW;Porcelijn, L;van Esch, WJE;Wuhrer, M;van der Schoot, CE;Vidarsson, G;Kapur, R;Year2020JournalBlood AdvPubMed ID: 32810222FoldedExpand
If you couldn"t find the specific citation, you can:
- Find All Citations
- Submit a CitationGet a Publication Reward
- Leave Us a Message
E.coli protein expression service
Mammalian transient protein expression service
Baculovirus-insect expression service
Stable cell line development service (CHO / HEK293)
品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
联络我们
