Sino Biological/MICA蛋白，人，重组（ECD，His-Tag）/1/12302-H08H
商品编号：
12302-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
MICA Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 97 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized MICAh (Cat: 12302-H08H1) at 2 μg/mL (100 μL/well) can bind S4-Fc3L3-NKG2D (Cat: 10575-H01S), the EC50 of S4-Fc3L3-NKG2D (Cat: 10575-H01S) is 120-300 ng/mL.
Protein Construction
A DNA sequence encoding the human MICA (AAH16929.1) extracellular domain (Met 1-Gln 308) was fused with a polyhistidine tag at the C-terminus.
Accession#
AAH16929.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu 24
Molecule Mass
The recombinant human MICA consists of 296 amino acids and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human MICA is approximately 45-65 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
MICA Protein, Human, Recombinant (ECD, His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized MICAh (Cat: 12302-H08H1) at 2 μg/mL (100 μL/well) can bind S4-Fc3L3-NKG2D (Cat: 10575-H01S), the EC50 of S4-Fc3L3-NKG2D (Cat: 10575-H01S) is 120-300 ng/mL.
MICA Protein, Human, Recombinant (ECD, His Tag): Synonyms
DAMA-345G11.2 Protein, Human; FLJ36918 Protein, Human; FLJ60820 Protein, Human; MGC111087 Protein, Human; MGC21250 Protein, Human; MIC-A Protein, Human; PERB11.1 Protein, Human
MICA Background Information
MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to the MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules do not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gamma-delta T cells, CD8+ alpha-beta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by the ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gamma-delta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.
Full Name
MHC class I polypeptide-related sequence A
References
- Choy MK, et al. (2010) MICA polymorphism: biology and importance in immunity and disease. Trends Mol Med. 16(3): 97-106.
- Li J, et al. (2005) Distinct pattern of human Vdelta1 gammadelta T cells recognizing MICA. Cell Mol Immunol. 2(4): 253-8.
- Mangham DC, et al. (2000) MICA-a highly sensitive and avidin-free immunohistochemical detection system. Adv Anat Pathol. 7(6): 360-4.
- Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428): 727-9.
- Groh V, et al. (1998) Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science. 279: 1737-40.
Human MICA Proteins
- MICA Protein, Human, Recombinant (His Tag)12302-H08H1
- MICA Protein, Human, Recombinant (His Tag), HPLC-verifiedHPLC-12302-H08H
- MICA Protein, Human, Recombinant (ECD, hFc Tag)12302-H02H
Cynomolgus MICA Proteins
- MICA Protein, Cynomolgus, Recombinant (ECD, His Tag)90924-C08H
- Targeted Activation of Human Vγ9Vδ2-T Cells Controls Epstein-Barr Virus-Induced B Cell Lymphoproliferative DiseaseAuthorZ Xiang, Y Liu, J Zheng, M Liu, A Lv, Y Gao, H Hu…Year2014JournalCancer cellApplicationcell-basedPubMed ID: 25220446FoldedExpand
- Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitisAuthorWang, F;Peng, PL;Lin, X;Chang, Y;Liu, J;Zhou, R;Nie, JY;Dong, WG;Zhao, Q;Li, J;Year2017JournalOncotargetApplicationcell-basedPubMed ID: 29228739FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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