Sino Biological/Podoplanin蛋白，小鼠，重组（His&；hFc标签）/1/50256-M03H
商品编号：
50256-M03H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Podoplanin Protein, Mouse, Recombinant (His & hFc Tag): Product Information
Purity
> 97 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Immobilized mouse PDPN-Fch at 10 μg/ml (100 μl/well) can bind biotinylated human CLEC1B-His (Cat:10976-H07H), The EC50 of biotinylated human CLEC1B-His (Cat:10976-H07H) is 0.04-0.08 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Leu 141) of mouse PDPN (NP_034459.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Accession#
NP_034459.2
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Gly 23
Molecule Mass
The recombinant mouse PDPN/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 367 amino acids and has a predicted molecular mass of 40.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmPDPN/Fc monomer is approximately 60-65 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Podoplanin Protein, Mouse, Recombinant (His & hFc Tag): Images
Immobilized mouse PDPN-Fch at 10 μg/ml (100 μl/well) can bind biotinylated human CLEC1B-His (Cat: 10976-H07H), The EC50 of biotinylated human CLEC1B-His (Cat: 10976-H07H) is 0.04-0.08 μg/ml.
Podoplanin Protein, Mouse, Recombinant (His & hFc Tag): Synonyms
Gp38 Protein, Mouse; OTS-8 Protein, Mouse; RANDAM-2 Protein, Mouse; T1-alpha Protein, Mouse; T1a Protein, Mouse; T1alpha Protein, Mouse
Podoplanin Background Information
Podoplanin, also known as PDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
podoplanin
References
- Kimura,N. et al., 2005, Pathol Int.55(2): 83-86.
- Ordóñez,N.G., 2006, Adv Anat Pathol.13(2): 83-88.
- Wicki,A. et al., 2007, Br. J. Cancer. 96(1): 1-5.
- Fujii,T.et al., 2008, Mod Pathol.21(2): 125-130.
- Sawa,Y.et al., 2008, Acta Histochem Cytochem.41(5):121-126.
- Kaddu,S. et al., 2009, Am J Dermatopathol.31(2): 137-139.
Human Podoplanin Proteins
- Podoplanin Protein, Human, Recombinant (His Tag)11065-H08H
- Podoplanin Protein, Human, Recombinant (ECD, His & hFc Tag)11065-H03H
Mouse Podoplanin Proteins
- Podoplanin Protein, Mouse, Recombinant (His Tag)50256-M08B1
Rat Podoplanin Proteins
- Podoplanin Protein, Rat, Recombinant (ECD, hFc Tag)81154-R02H
- C-type lectin-like receptor 2 (CLEC-2)-dependent DC migration is controlled by tetraspanin CD37Authorde Winde, CM;Matthews, AL;van Deventer, S;Year2017JournalbioRxivApplicationcell-basedPubMed ID: 30185523FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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