Sino Biological/M-CSF Protein, Human, Cynomolgus, Recombinant (His Tag)/1/11792-H08H
商品编号：
11792-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
M-CSF Protein, Human, Cynomolgus, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its binding ability in a functional ELISA.2. Immobilized human CSF1R (Cat:10161-H08H) at 10 μg/mL (100 μl/well) can bindbiotinylated human CSF-1 (Cat:11792-H08H).The EC50 of biotinylated human CSF-1 (Cat:11792-H08H) is 8 ng/mL.3. Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is typically 2-8 ng/mL.
Protein Construction
A DNA sequence encoding the N-terminal fragment (Met 1-Asn 190) of human / cynomolgus CSF1 (P09603-1) was fused with a polyhistidine tag at the C-terminus. Human and Cynomolgus CSF1 sequences are identical.
Accession#
P09603-1
Expressed Host
HEK293 Cells
Species
Human, Cynomolgus
Predicted N Terminal
Glu 22
Molecule Mass
The recombinant human / cynomolgus CSF1 consists of 169 amino acids and predictes a molecular mass of 19.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCSF1 is approximately 27-32 kDa band due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
M-CSF Protein, Human, Cynomolgus, Recombinant (His Tag): Images
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is typically 2-8 ng/mL.
M-CSF Background Information
Macrophage colony-stimulating factor1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membraneprotein which is disulfide-linked as a homodimeror heterodimer. Granulocyte / macrophagecolony-stimulating factors are cytokines that act in hematopoiesis by controlling the production,differentiation, and function of 2 related white cell populations of the blood,the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known tofacilitate monocyte survival, monocyte-to-macrophage conversion, andmacrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate intomacrophages or other related cell types. It binds to the Colony stimulatingfactor 1 receptor. M-CSF/CSF-1 may also be involved in development of theplacenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-boundprecursors. M-CSF/CSF-1 induces cells of the monocyte/macrophagelineage. It also plays a role in immunological defenses, bone metabolism,lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarctedmyocardium may have an active role in healing not only through its effects oncells of monocyte/macrophage lineage, but also byregulating endothelial cell chemokine expression.
Full Name
colony stimulating factor 1 (macrophage)
References
- Pandit J. et al., 1992, Science. 258: 1358-62.
- Tokai M. et al., 2000, J Bacteriol. 182(10): 2865-8.
- Fan X. et al., 2001, Am J Physiol Endocrinol Metab. 280(1): E103-11.
- Frangogiannis NG. et al., 2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92.
- Cupp JS. et al., 2007,Am J Surg Pathol. 31(6): 970-6.
Human M-CSF Proteins
- M-CSF Protein, Human, Recombinant (His Tag)11792-H08Y
- M-CSF Protein, Human, Recombinant, Biotinylated11792-HNAH-B
- M-CSF Protein, Human, Recombinant11792-HNAH
- M-CSF Protein, Human, Recombinant (hFc Tag)11792-H02H
- M-CSF Protein, Human, Recombinant (His Tag)11792-H08H1
Mouse M-CSF Proteins
- M-CSF Protein, Mouse, Recombinant (ECD)51112-MNAH
- M-CSF Protein, Mouse, Recombinant (His Tag)51112-M08H
Human, Cynomolgus M-CSF Proteins
- M-CSF Protein, Human, Cynomolgus, Recombinant (His Tag), Biotinylated11792-H08H-B
Cynomolgus, Rhesus M-CSF Proteins
- M-CSF Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)90029-C02H
- M-CSF Protein, Cynomolgus, Rhesus, Recombinant (His Tag)90029-C08H
- A potent in vivo anti-tumor efficacy of novel recombinant type I interferonAuthorZhang, K;Yin, XF;Yang, YQ;Li, HL;Xu, YN;Chen, LY;Liu, XJ;Yuan, SJ;Fang, XL;Xiao, J;Wu, S;Xu, H;Chu, L;Katlinski, KV;Katlinskaya, YV;Guo, RB;Wei, GW;Wang, DC;Liu, X;Fuchs, SY;Year2016JournalClin. Cancer Res.ApplicationbindingFoldedExpand
- A potent in vivo anti-tumor efficacy of novel recombinant type I interferonAuthorZhang, K;Yin, XF;Yang, YQ;Li, HL;Xu, YN;Chen, LY;Liu, XJ;Yuan, SJ;Fang, XL;Xiao, J;Wu, S;Xu, H;Chu, L;Katlinski, KV;Katlinskaya, YV;Guo, RB;Wei, GW;Wang, DC;Liu, X;Fuchs, SY;Year2016JournalClin. Cancer Res.ApplicationbindingFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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